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Identify and provide either an concise explanation of the concept signal-to-noise ratio (SNR), in the context of chemical analysis.
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- The use of statistics in analytical chemistry has become important and essentials in recent years. With specific examples, discuss four practical applications and/or utility of statistics in analytical chemistry.Given the equation of a calibration curve y=5.63x + 0.006, identify the variable that equates to the absorbance value of the Beer's law equation.In order to measure riboflavin in a breakfast cereal, a 5.00-gram sample of the cereal was extracted with acetic acid and filtered. The extract was transferred to a 50.0 mL volumetric flask and diluted to the mark. The sample was transferred to a cuvette and the fluorescence was measured. The instrument read 59 units (Fluorescence units –an arbitrary scale). 24.00 mL of the cereal extract was sample was mixed with 1.0 mL of a riboflavin standard that was 0.500 ug/mL. The fluorescence of this mixture was measured and read 94 fluorescence units. Calculate the concentration of riboflavin per gram of cereal (use µg/gram of cereal).
- Discuss thoroughly two (2) spectroscopic techniques used in analytical chemistry.Suppose a student is preparing a set of standard solutions for use in a spectrophotometric study. If percent transmittance, %T, were measured in the standard solutions before the color had a chance to fully develop, the measured value would be compared with solutions that have allowed the color to fully develop.Distinguish between the terms elution and eluent.
- 1-31. We shall learn when we study molecular spectroscopy that heteronuclear diatomic molecules absorb radiation in the microwave region and that one determines directly the so-called rotational constant of the molecule defined by B = 8721 where I is the moment of inertia of the molecule. Given that B = 3.13 × 105 MHz for H35C1, calculate the internuclear separation for H35C1. The atomic mass of H and 35C1 are 1.008 and 34.97, respectively.List the essential parts of a spectrophotometer. Hence write the working principles or functions for each component.A 600 MHz NMR has a signal to noise ratio of 1000 for a proton standard. The 400 MHz NMR has a signal to noise ratio of 200 for a proton standard. If it takes 20 minutes to get an acceptable signal to noise ratio for a sample on the 600 MHz NMR, how long will it take you to achieve the same signal to noise ratio on the 400 MHz NMR?
- What is the modulated wavelength of mercury vapor light at a temperature of 88°F and barometric pressure of 725 mmHg, known that the frequency of modulation is 30 MHz?3. The change in frequency produced by the Doppler shift when a sound source of frequency f is moving with velocity v toward a fixed observer is given by fv Af (u-v) and v and their uncertainties, calculate Af and its uncertainty. Which, if any, of where u is the velocity of sound. From the following values of u, f, the uncertainties make a negligible contribution to the uncertainty in Af ? u = (332 ± 8) m s1; f = (1000 ± 1) Hz; and v = (0.123 ± 0.003) m s1The following data have been obtained in the calibration of a series of standards for the determination of iron, measuring the transmittance, at 506 nm and thickness of 1.00 cm of iron (II) solutions in which color with 1.10 has been developed -phenanthroline. [Fe] (ppm) 0.20 0.40 0.60 0.80 1.00 2.00 3.00 4.00 5.00 6.00 7.00 T (%) 90.0 82.5 76.0 69.5 63.5 41.0 26.3 17.0 10.9 7.0 4.5 Calculate the mean molar absorptivity of iron when determined by this method.