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How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)
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- What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock concentration of 10 uM in a total volume of 1 mL?A buffer contains 0.015 mol of lactic acid (pK₁ = 3.86) and 0.080 mol of sodium lactate per liter. H₂C OH Lactic acid OH Calculate the pH of the buffer. H₂C. Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of the buffer. O OH Sodium lactate Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of pure water. O Na+ buffer pH: buffer pH change: water pH change: units unitsCalculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.
- I want to make a buffer solution containing 1 M glycene pH = 10 (Na+), 1 mM ZnCl2 , 1 mM MgCl2. I have the stock solutions 1 M glycene, 1 M MgCl2, 1 M ZnCl2, and 10 M NaOH. My final volume of buffer solution needs to be 250ml. how much (in ml) of each solution should i put into my buffer?You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.What is the pH of a buffer that contains 0.9 M ammonia and 0.3 M ammonium ion? (Ka for ammonium ion = 5.8 x 10-10
- What is the final concentration if 239mL of a 3.9M glucose solution is diluted to a volume of 842mL?You are supplied with the following: NaCl (Mr = 58.443 g/mol) 250 mM Tris-Cl, pH 8 solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 13 % Sodium dodecyl sulfate solution Proteinase K solution (150 mg dissolved in 1 ml ddH2O) You need a digestion buffer consisting of the following: 23.5 mM NaCl 750 uM Tris-Cl, pH 8 38 mM EDTA, pH 8 1.2 % Sodium dodecyl sulfate 1.75 mg/ml proteinase K Calculate: How will you prepare 750 ml of the digestion buffer? Show all your steps and calculations and remember to explain how exactly you will make it up.What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4
- You have just used up the last of a 1.2 % m/m solution of agarose in water. You require another 100mls. How many grams of Agarose do you require? Note 1ml of water=1g What is the % w/v of a 100ml solution containing 5g of NaCl?What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.Calculate the pH of the resulting solution if 23.0 mL of 0.230 M HCl(aq) is added to 33.0 mL of 0.230 M NaOH(aq). pH = Calculate the pH of the resulting solution if 23.0 mL of 0.230 M HCl(aq) is added to 13.0 mL of 0.330 M NaOH(aq). pH=