Hexokinase in red blood cells has a Km of approximately 50 µM. What concentration of blood glucose would yield a velocity equal to 90 % Vmax?
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- Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires 3500xg, what would your setting be in a given centrifuge with a radius of 10cm? Provide computationsUse MS Excel to plot and determine the terms being asked: Please insert the excel file, thank you For each V (mmol/min), determine the value of [S] (mM) if Vmax = 5 mmol/min. Show your calculations. Using MS Excel, plot [S] (mM) versus V (mmol/min). Draw a line corresponding to the given Vmax parallel the x-axis and extend your plotted line to show its approach to Vmax. Provide your final plot [S] (mM) V (mmol/min) 10 1.2 1.7 2.1 2.2 2.5 Determine the values of Km and Vmax for the decarboxylation of β-keto acid given the following data. Please show and label your plot. Show your calculations and box your final [S] in mol/l V in mM/ min 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256What is the Keq for the conversion of Glucose 6-Phosphate to Glucose 1-Phosphate if the phosphate transfer potential for Glucose 1-Phosphate and Glucose 6-Phosphate are -20.9 kJ/mol and -13.8 kJ/mol respectively? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 0.27 b 0.45 c 10.5 d 0.019 e 0.057 f 24.2 g 7.10 h 0.24 i 2477
- A biochemist is trying to determine the type of proteases they have isolated from walrus blubber. The three proteases and their relative activities in the presence of the indicated non-specific irreversible inhibitors are shown in the table below: Protease a Protease B Protease y Given this data, please answer the following question: The catalytic site of Protease ß contains an important: C R H + lodoacetate Normal Activity Normal Activity No Activity U + Tetranitromethane Normal Activity No Activity Normal ActivityYou plot a reaction progress curve and find the equation of the line to be y = -0.0961x + 0.7142. Calculate the U, U/μL and U/mL of lysozyme activity in this fraction. The lysozyme activity is done identical to what is indicated in the lab manual.The standard curve provided below will allow you to answer the remaining questions. Generic Standard Curve 0.7 y = 0.9425x- 0.0034 0.6 0.5 0.4 0.3 02 0.1 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Concentration (mg/ml) - Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample.| #1: #2: -What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150? Absorbance
- A glucose solution is supplied in a repeated fed batch culture. The following parameters have been measured at t=2.5 hours; the system was under a quasi-steady state at this time. V = 1.0L, F = 0.22L/hr, S_0 = 98g/L, mu_m = 0.31/hr, K_s = 0.14 g-glucose/L, YM_X/S = 0.48 g-cells/g-glucose, Xt_0 = 33g (1) Calculate the value of V0 and S. (2) Calculate the value of Xt for t = 2.5hrs.Neutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of enzyme is it? Assume Vmax is 35 µM min-1. When you provide 3.0 x 10-5 M of sphingomyelin, you observe an initial velocity of 6.0 µM min-1. Calculate the KM.If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02 sec/mol, and x-intercept is -2.5 mM-1, then what is the KM value?
- What is the x-intercept for the above reaction on a Lineweaver-Burk plot?A. -0.5 per mMB. -1.0 per mMC. -2.0 per mMD. -5.0 per mME. -10.0 per mMA flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?The enzyme phosphoserine phosphatase catalyses the reaction: phosphoserine + H2O → serine + Pi Kinetics measurements were performed using a range of Mg* concentrations and in the presence of different fixed concentrations of Ca-". The enzyme requires Mg²* for activity. The following data were recorded: [Ca²*] / mM 0.0 20.0 40.0 [Mg*]/ mM Initial velocity / µmol of product min 0.33 20.40 9.26 5.99 0.50 25.65 12.66 8.47 1.00 33.30 20.00 14.28 2.00 40.00 28.57 22.23 What type of inhibitor is Ca2* and what is its Kf? Suggest what role Mg** performs in the catalytic mechanism of phosphoserine phosphatase. Suggest how Ca2* inhibits the reaction.