help me with this multiple choice question please, this is molecular and cell biology Visualization of an electrophoresed agarose gel (D) Plasmid DNA cannot be visualized on an agarose gel (A) Plasmid DNA may be visualized as multiple supercoiled forms (B) Visualized plasmid DNA is always linear Ⓒ(C) Visualized plasmid DNA is always circular

help me with this multiple choice question please, this is molecular and cell biology Visualization of an electrophoresed agarose gel (D) Plasmid DNA cannot be visualized on an agarose gel (A) Plasmid DNA may be visualized as multiple supercoiled forms (B) Visualized plasmid DNA is always linear Ⓒ(C) Visualized plasmid DNA is always circular

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter16: Adult And Pediatric Dosages Based On Body Surface Area

Section: Chapter Questions

Problem 7.2P

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Hi, this is my Recombinant "Paper" Plasmid activity question, I did other parts, but this question I really have no clue what it is. Question: What polypeptide sequence does the inserted gene code for? (Review protein synthesis and write the full amino acid sequence for the inserted gene.) in the picture attached: I Draw a ring symbolizing my plasmid that has been recombined with another gene. it shows the following: • where inserted gene is located on your loop. • where the origin of replication is located. • which antibiotic resistant genes (R – genes) I preserved and what sequential order is • Name and where your restriction enzymes (endonucleases) cut.
Activity 2. You COMPLETE Me! Objective: Identify the methods used for inserting plasmids into the host cells. What you need: pen and paper What to do: On a separate sheet of paper, copy and complete the key points of the methods used to introduce plasmids into the host cells. Choose your answers from the box gene gun biolistics electric shock mammalian cells plasmid insertion by Heat Shock Treatment increase the pore sizes of their plasma membranes (2) competent plasmid DNA Heat Shock electroporation There are three methods on introducing plasmids into the host cells namely: (1)_ (3) Biolistics uses a (4)_ to fire DNA-coated pellets on plant tissues. Plasmid insertion by Heat Shock Treatment is a process wherein target cells undergo a The pretreatment is said to make the cells (6). pretreatment procedure to (5) the introduction of the (7)_ plasmid at about 4°C for about 30 minutes and the plasmids concentrate near the cells during After the pretreatment, the cells are incubated with…
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Help Please Supercoiled DNA migrates through an agorose gel faster than similarly sized linear DNA? True or False? Using the plasmid map, indicate where (bp region on plasmid) these enzymes cut on the plasmid. EcoR1 1. 1 Hindlll 2. 637 Bpu101 3. 14 BamHI 4. 256 To sequence a DNA plasmid, you need 1 µg of DNA. If your DNA concentration was 0.45 µg/µL. How many µl of your DNA 0.45 µg/µL is required to get 1 µg final? Answer in µl using 2 decimal places. Your Answer: Answer units >
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1 (a) What do ApR, TcR, and ori on the pBR322 map represent and discuss there individual functions? (b)Does the undigested plasmid show more than a single band when electrophoresed? Why? (c)What other kinds of molecules, in addition to plasmid DNA would you expect to find in a sample of plasmid DNA extraction? (This is not a graded assignment)
Homogenize cells Centrifuge Heat-kill Recover IIIS filtrate IIIS cells spun to bottom of tube Extract carbohydrates, lipids, and proteins IIIS cells in liquid culture medium Treat with ribonuclease Treat with Treat with deoxyribonuclease protease Assay for Transformation IIR cells IIR cells IIR cells IIR cells + IIIS filtrate DNase-treated RNase-treated IIIS filtrate Protease-treated IIIS filtrate IIIS filtrate No transformation Transformation Transformation Transformation occurs occurs occurs occurs IIR cells IIR cells IIR cells Only IIR cells + IIIS cells IIIS cells IIIS cells Conclusion: Active factor is DNA Conclusion: Active factor is not RNA Conclusion: Active factor is Control: IIIS contains not protein active factor E IIIS cells EA IIR cells 8. For each molecule listed, indicate whether they caused a transformation (Yes) or did not cause a transformation (No): a. RNA b. DNA c. Proteins yes d. Carbohydrate e. Lipids
Animal Cell DNA Bacterial cell Plasmid Bacterial 1 3 5 chromosome Questions: 1. Why are plasmids used as vector for DNA Recombination? What other vectors can be used? 2. How are Recombinant DNA formed? 3. What is the difference between genetic modification and selective breeding? EXCELLE
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Practice Case study p. 224 part: Tuberculosis (TB) in indigenous populations. Lily was a recent science graduate of the University of Alberta and was 11 months into her 12 - month Master of Science in Global Health degree, at Dalhousie University. This relatively new program was created to provide the multidisciplinary training required of scientists working to meet the complex challenges of global public health issues. The University had partnered with the National Collaborating Centre for Indigenous Health (NCCIH) and that was why Lily now found herself doing her Global Health Field Practicum in the Northern Territories, working on a study of drug resistant TB infections among Innuit communities. The different communities included in her research were close to 7000 individuals, with multiple diagnosed cases of TB. And while she was grateful that drug-resistant TB in Canada remained below the global average (Public Health Agency of Canada, 2019), she was cognizant of the possibility…
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