When cells undergo apoptosis, their DNA becomes fragmented. When the DNA is run out on a gel, the resulting fragments differ in size by a standard amount, about 200 bp, so that the resulting separated DNA has a characteristic "ladder" appearance.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
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When cells undergo apoptosis, their DNA becomes
fragmented. When the DNA is run out on a gel, the
resulting fragments differ in size by a standard
amount, about 200 bp, so that the resulting
separated DNA has a characteristic "ladder"
appearance.
Control
Staurosporine
treated
Transcribed Image Text:When cells undergo apoptosis, their DNA becomes fragmented. When the DNA is run out on a gel, the resulting fragments differ in size by a standard amount, about 200 bp, so that the resulting separated DNA has a characteristic "ladder" appearance. Control Staurosporine treated
Explain why this pattern of DNA fragments is obtained, based on what you know about eukaryotic chromatin.
Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used.
20 bp
one nucleosome
regular
200 bp
146 bp
two histone pairs
random
50 bp
The DNA wrapped around a histone core is
2
linker region, then the DNA will be cut at
vary in size by
multiples of 200 bp.
If the caspase-stimulated DNase cuts the DNA at
Reset Help
and the linker is approximately
intervals within the
along the chromatin, yielding fragments that
per linker DNA repeat. This leads to fragments that differ in size by
Transcribed Image Text:Explain why this pattern of DNA fragments is obtained, based on what you know about eukaryotic chromatin. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. 20 bp one nucleosome regular 200 bp 146 bp two histone pairs random 50 bp The DNA wrapped around a histone core is 2 linker region, then the DNA will be cut at vary in size by multiples of 200 bp. If the caspase-stimulated DNase cuts the DNA at Reset Help and the linker is approximately intervals within the along the chromatin, yielding fragments that per linker DNA repeat. This leads to fragments that differ in size by
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