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Draw a schematic diagram for the preparation of broth media in a laboratory set-up.
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- Explain when "formulated media" was chosen to be used as a medium in the fermentation process? Mention two examples of formulated media!explain how reducing media decrease the concentration of oxygen in the media when there is oxygen everywhere in the environment/surrounding atmosphere.Explain the purpose of thiosulfate in the medium.
- What tests would you use to determine if an organism was fermentation positive or negative? Include in your answer the indicator you would use. In addition to carbohydrate, what other major organic material, present in the medium, could be broken down to produce energy and what would be the final color change be used when it is broken down?. Also indicate the three possible color changes of the indicator used and the three pH levels at which the medium changes color. Explain the purpose of the Durham or fermentation tube in the test tube and why it is upside down? What gas is produced in fermentation of glucose? The purpose of the fermentation tests are to determine members of what family from other gram negative organisms? Name a genera of Gram positive cocci from a genera of Gram positive cocci which is a non-fermenter.Identify which type of plant is suitable to produce the Biogas at constant pressure? Discuss its working operation?Arrange the following steps in the experiment regarding the effect of temperature on fermentation (anaerobic respiration) 1 4 Sugar and water are mixed Measure the height of foam is measured Test tubes are placed in water bath Yeast and glucose solution are mixed Graph the height vs temperature