Answered: determine the sequence of the mystery…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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3. Solve the sequence of an oligopeptide 7 residues long which gave: Asp Leu Lys Met Phe Tyr The following facts were observed: a. Trypsin treatment had no apparent effect b. The PTH derivative from Edman degradation was c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met. d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys. Instructions Make use of the table below to determine the sequence of the mystery protein.

Reagent
Cleavage Points
Trypsin
Lys, Arg (C)
Submaxillary protease
Arg (C)
Chymotrypsin
Phe, Trp, Tyr (C)
2/4
Staphylococcus aureus
Asp, Glu (C)
Asp-N-protease
Asp, Glu (N)
Pepsin
Leu, Phe, Trp, Tyr (N)
Endoproteinase Lys C
Lys (C)
Cyanogen bromide
Met (C)
Carboxypeptidase A
Works on all residues (C) except Pro, Arg, Lys,
Asp, and Glu
Carboxypeptidase B
Arg, Lys (C)

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