Derivatiztion of sample is carried out to: a) Improve analyte peak shape analyte resolution b) Increase the detector response c) Improve d) All the above
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- 8. Derivatiztion of sample is carried out to: a) Improve analyte peak shape analyte resolution b) Increase the detector response c) Improve d) All the aboveRetention time is the time taken for the mobile phase to reach the detector. True FalseWhat is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly answer at your own words. Answer should be to the point (4-5 lines).
- This method is to be used when measuring the volume of a solution containing sufficient reagent to react completely with the analyte. Group of answer choices Volumetric Spectroscopic Gravimetric ElectroanalyticalAnalysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min respectively in a 30.0 cm column. The peak base width for X is 1.06 and 1.15 for Y. The average number of plates in the column isAnalysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min respectively in a 30.0 cm column. The peak base width for X is 1.06 and 1.15 for Y. The analysis gave a column resolution equal to?
- 5. Using the diagram answer the questions below: Increasing time Sample applied p to top of column Column fow (a) eluent eluent eluent eluent (A) Draw a sketch of the expected chromatrogram (peaks, broadening, relative elution times, etc.) (B) Explain the term "analyte dilution" using the diagram as a reference.of the analyte increases, As the temperature of a gas chromatograplhy column increases, the the retention time , and the peak shape Select one: O a. temperature; increases; sharpens O b. vapor pressure; decreases; sharpens O c. equilibrium; increases; broadens O d. vapor pressure; increases; sharpens O e. solubility; decreases; sharpensIn chromatography, it is desirable to increase the resolution. a) increase the plate height (H) b) decrease the plate height (H) c) decrease the number of plates (N) to
- SPECTROPHOTOMETRY 1.) Slope of regression line or Molar absorptivity (µM -1cm-1) is 0.0253 and absorbance of unknown solution is 0.131. What is the concentration of the unknown solution (µM )?A method of quantitative determination of the concentration of constituentsof a sample analyzed by gas chromatography is area normalization. Thiscomplete elution of all sample constituents is required. Thearea of each peak is measured and corrected for the detector response to the diffe- renteseluatos. This correction involves dividing the area by an empiricalcorrection factorDetermined. The concen ration of the analyte is found from the ratio between itscorrected and the total corrected area of all peaks. For a chromatogram containing threerelative areas were determined as 16.4; 45,2; and 30.2, in the order ofincreased retention time. Calculate the percentage of each compound if therelative responses from the detector are 0.65, 0.73 and 0.85, respectively.Why is Spooled a better estimate of s than the standard deviation from any one sample?