Calculate the signal attenuation along a neuron of radius 5 um after the signal has travelled a distance 100 um. The ratio of the axoplasma resitivity to the wall resiativity is 0.01.
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Calculate the signal attenuation along a neuron of radius 5 um after the signal has travelled a distance 100 um. The ratio of the axoplasma resitivity to the wall resiativity is 0.01.
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- Regarding the passive electrical properties of a neuron, calculate how the EPSP will be affected as determined by the length and time constants, when the ability of the cell membrane has a capacity of charge separation of 75% and a resistance of 60 %. The student investigating the EPSP has determined that the region of the dendrite in which the electrode shows an internal resistance of 0.5 due the diameter of this dendrite proximal to the soma.Patch clamp recording of a single ion channel yields the following results: Holding Potential (mV) Measured Current (pA) -100 -1.0 -50 0.0 0 +1.0 +50 +2.0 +100 +3.0 part a.) Calculate the membrane potential at the instant when a neuron has the following relative permeabilities: PK+ = 1.0, PNa+ = 1.0, PCl- = 1.0. Use the ionic concentration values in the picture included. a.) -12 mv b.) -35mv c.) -60 mv d.) +20 mv e.) 0 mv part b.) What would be the equilibrium potential for K+ in neurons under such circumstances? a.) -11 mv b.) +30 mv c.) +75 mv d.) -35 mv e.) 0 mv part c.) What would be the new resting potential, discounting the effects of non-gated chloride channels (just give an approximate value – no calculation is necessary)? a.) about +20 to +25 mV b.) about -30 to -40 mV c.) about +1 to +5 mV d.) close to ENa+ e.) about -10 to -15 mVYou are recording from a cell with a resting membrane potential of -65 mV. You inject 100 pA of current, resulting 58.7 mV. What is the value of tau? With this in mind, what is the capacitance of the cell? Please provide relevant equations to illustrate your thought process. When you terminate the current injection, how long do you expect for it to take for the cell to repolarize to -63 mV?
- Assume the membrane is only permeable to Na+ and K+, the electrical model of axon membrane is the following: Outside A INa gna ENa Ex D Inside The membrane potential at rest and at peak is -70mV and 45mV respectively; the potential of Sodium ion is 60mV and the potential of Potassium ion is -80mV. Find the ratio 9Na at rest and at peak. gKCompound X has been shown to block voltage-gated K+ channels with an IC50 of 0.1 mM. Which of the following is the most likely to occur in neurones following application of 0.03 mM X? Action potentials would be prolonged and the resting potential would be depolarised. Action potentials would be prolonged but the resting potential would remain the same. Neither resting potential nor action potentials would be affected because the concentration of X is less than the IC50 value. The resting membrane potential would depolarise but there would be no effect on action potentials.Draw the current that you would expect to flow during a voltage clamp experiment on a typical neuron. Voltages and time course are shown. Briefly explain why the currents are inward or outward. Be sure to provide scale bars. You should definitely label the Y axis so that the peak current value is obvious. Draw the Na+ current you would expect if there were physiological ionic gradients. Draw the K+ current you would expect if there are physiological ionic gradients. Draw the K+ current you would expect if the bath solution and the intracellular solution are both 125 mM.
- You are obtaining extracellular recordings from the latetal axons of the earth worm. The distance from the stimulating cathode to the first recording electrode is 60mm and 85mm to the second recording electrode. The time from stimulation to arrival of the action potential at the first recording electrode is 5msec and to the second recording electrode is 10msec. The conduction velocity of this axon would be meters per second. 05 0.2 Ⓒ10 3What is the expected resting membrane potential (in mV) of a neuron that is typical in all ways except for possessing an extracellular potassium concentration of 104.3 mM, an intracellular sodium concentration of 103.6 mM, and 4 times the normal resting permeability to sodium?For a nerve fibre axoplasmic and extracellular ion concentrations were found to be respectively: Na+, 15 and 115 mM; K+, 90 and 3 mM; Cl-, 10 and 120 mM. Resting potential was -78 mV. Halving the external [Na+] caused a very slight hyperpolarization; doubling external [K+] caused considerable depolarization; halving the external [Cl-] had no effect. In each case the ions were replaced by impermeable salts. What can you deduce (with reasons) about the resting membrane conductances?
- What is the intracellular voltage for Cl- if the intracellular concentration was 5 mM and the extracellular concentration was 130 mM. Write the voltage as a number in mV inside the cell relative to that outside, eg. -72 or + 90. Question 2. What is the intracellular voltage for Ca++ if the intracellular concentration was 0.0008 mM and the extracellular concentration was 0.6 mM. Write the voltage as a number in mV inside the cell relative to that outside, eg. -72 or +90. Round to the nearest milli Volt.Draw details of the repolarization phase of an action potential from the following descriptions of the sequences of AfterHyperPolarization (AHP) and AfterDePolarization (ADP) sequences. Make the distinct phases clear and noticeable (5 % each) A complex AHP consisting of a first component AHP, an ADP, and a second component AHP before repolarization to resting membrane potential a first fast AHP component, followed by a slower AHP, followed by a fast ADP, and a second late AHP component before repolarization to restTwo branching dendritic arbors are stimulated at the same exact time at locations A & B which are both 10 um away from the axon hillock. Upon stimulation, location A depolarizes 20 mV and location B depolarizes 15 mV. The time constant is the same in both dendrites. The length constant in both dendrites is 10 um. The RMP is -70 mV and the threshold to fire an action potential is -55 mV. Based on the above information, does the neuron fire an action potential? Explain your answer.
