Biology

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
Problem 1P
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Give the recognition sequences for each of the restriction enzymes (a) PagI, (b) AluI, (c) PstI, and (d) RcaI. Show the sequence in double-stranded DNA (dsDNA) format, indicate the cleavage position with a ^, and mention which types of ends are generated in each case (blunt or sticky; 5’ or 3’ overhang)?

Recognition sequence for  a)PagI cleaves DNA at the recognition sequence 5' ---T ^CATGA--- 3'  b) AluI cleaves DNA at the recognition sequence 5' AG^CT 3' c) PstI cleaves DNA at the recog.

Biology
Give the recognition sequences for each of the
restriction enzymes (a) Pagl, (b) Alul, (c)
Pstl, and (d) Rcal. Show the sequence in double-
stranded DNA (dsDNA) format, indicate
the cleavage position with a ^, and mention
which types of ends are generated in each case
(blunt or sticky; 5' or 3' overhang).
Transcribed Image Text:Biology Give the recognition sequences for each of the restriction enzymes (a) Pagl, (b) Alul, (c) Pstl, and (d) Rcal. Show the sequence in double- stranded DNA (dsDNA) format, indicate the cleavage position with a ^, and mention which types of ends are generated in each case (blunt or sticky; 5' or 3' overhang).
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