Based on the knowledge you gained from the cloning module, which of the bands in gure is associated with a supercoiled plasmid?
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- Based on the knowledge you gained from the cloning module, which of the bands in the figure is associated with a supercoiled plasmid?Based on the knowiedge you gained from the cloning module. which of the lanes in the figure is associated with a digested plasmid that contained a PCR insert?Hi, this is my Recombinant "Paper" Plasmid activity question, I did other parts, but this question I really have no clue what it is. I attached a photo of the letters on the inserted gene code, please help me figure out what it means, Thanks a lot. It is the shaded region
- Based on the knowledge you gained from the cloning module, which of the bands in the figure is associated with the PCR insert after digestion of the plasmid?With reference to the image below, discuss the process and principle involved for screening/selection ofhosts (last stage of cloning) containing the intended recombinant plasmid.Sal1 Xho1 Insert pUC cloning vector MCS Insert Digested with Sal1 on the left and Xho1 on the right Sal1 Ori Vector Digested with Sal1 Xho1: СТСGAG Sal1: GTCGAC RE cut site Part B) After 8 h of sleep, you realize your mistake above (Question 3 Part A), and you successfully transformed your plasmid and obtained a subset of white colonies and blue colonies on the plate. Question: You conclude that all the blue colonies contain a plasmid O With the insert in the LacZ screening marker With the insert in the Antibiotic selectable marker O Without an insert in the LacZ screening marker O None of the above + Lacz alpha AmpR
- In biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. State the three (3) important regions of the plasmid. Elaborate your answer.After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that had X-gal and ampicillin. X-Gal is often used as in indicator dye, which turns blue when metabolized by B-galactosidase protein and used to test if cloning experiments have worked. [Note look at the vector diagrams carefully] Briefly explain how you would find the bacterial cells that are transformed with the plasmid with the YFG inserted.Amir has some wild type Vibrio sp. which he would like to mutate for his experiment. Priorto mutation, he must ensure that the Vibrio are able to take up foreign plasmid / DNA.Illustrate using diagrams the method he can perform and detail each step of the method.
- Clone number in this case is number 196 as shown in the images. What is the exact length of the segment of human DNA that has been inserted into the plasmid? *report the entire length of the insert, not just the sequences matching the ends and labels of wells isn't needed for answer*Hi, this is my Recombinant "Paper" Plasmid activity question, I did other parts, but this question I really have no clue what it is. Question: What polypeptide sequence does the inserted gene code for? (Review protein synthesis and write the full amino acid sequence for the inserted gene.) in the picture attached: I Draw a ring symbolizing my plasmid that has been recombined with another gene. it shows the following: • where inserted gene is located on your loop. • where the origin of replication is located. • which antibiotic resistant genes (R – genes) I preserved and what sequential order is • Name and where your restriction enzymes (endonucleases) cut.Using the plasmid map of pBCH2.0 calculate the length of the shortest DNA fragment if this plasmid was digested with the restriction enzymes PstI and EcoRV.