An extraction Buffer, EB(200 mM Trish-HCL(Ph 8), 250 mM NaCl, 25 mM EDTA, 0.5% SDS) is used for DNA extraction from cells. Use their stock solutions to prepare their respective working concentrations for the given working volume of EB. Stock Solutions are: 1M Tris-HCL(pH~8) 0.5M EDTA 0.8M NaCl 10% SDS Show final calculaed values in the table given below. Component 1L EB 500 ml EB 250ml EB 100ml EB 200mM Tris-Hcl 250mM NaCl 25mM EDTA 0.5% SDS dH2O

An extraction Buffer, EB(200 mM Trish-HCL(Ph 8), 250 mM NaCl, 25 mM EDTA, 0.5% SDS) is used for DNA extraction from cells. Use their stock solutions to prepare their respective working concentrations for the given working volume of EB. Stock Solutions are: 1M Tris-HCL(pH~8) 0.5M EDTA 0.8M NaCl 10% SDS Show final calculaed values in the table given below. Component 1L EB 500 ml EB 250ml EB 100ml EB 200mM Tris-Hcl 250mM NaCl 25mM EDTA 0.5% SDS dH2O

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter1: Relative Value, Addition, And Subtraction Of Decimals

Section: Chapter Questions

Problem 39SST

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To prepare a gel sample, you want to load 50 ng total of protein/well. You have added 200 μL of protein in 800 µL reagent and measured your sample by Bradford A595 to be 0.7 mg/mL - your dilution is unaccounted for at this point. Assuming a total final volume of 20 μL, what volume of protein sample, buffer and 4X SDS PAGE Loading Dye needs to be mixed to create a final sample of 50 ng protein in 20 μL?
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