A. Add mutagen to culture of Saccharomyces cerevisiae B. Add mutagen to plate C. Incubate to allow colonies to grow D. Plate Saccharomyces cerevisiae on solid media containing Leu E. Plate Saccharomyces cerevisiae on solid media lacking Leu Replica plate onto solid media lacking Leu F. G. H. I. Replica plate onto solid media containing Leu Identify colonies that did not grow on the replica plate Identify colonies that grew on the replica plate

A. Add mutagen to culture of Saccharomyces cerevisiae B. Add mutagen to plate C. Incubate to allow colonies to grow D. Plate Saccharomyces cerevisiae on solid media containing Leu E. Plate Saccharomyces cerevisiae on solid media lacking Leu Replica plate onto solid media lacking Leu F. G. H. I. Replica plate onto solid media containing Leu Identify colonies that did not grow on the replica plate Identify colonies that grew on the replica plate

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter17: Biotechnology And Genomics

Section: Chapter Questions

Problem 13RQ: Information obtained by microscopic analysis of stained chromosomes is used in: radiation hybrid...

See similar textbooks

Similar questions

/take S. pyogenes may contain deoxyribonuclease. What is the role of this enzyme? Deoxyribonuclease facilitates the production of DNA from bacterial cells. Deoxyribonuclease is an enzyme that replicates DNA and then proteins. O Deoxyribonuclease breaks down proteins and damages the cell. O Deoxyribonuclease breaks down DNA released from damaged cells. Question 4 Which organism can produce pyocyanin? MacBook Air
Use the following information to answer the next question. Human genes were integrated into the chromosomes of pig sperm using the procedure of sperm-mediated gene transfer (SMGT). In the first successful SMGT procedure, scientists used an enzyme to integrate three human genes into the chromosomes of pig sperm. Approximately 90% of the resulting pig embryos carried all three human genes in very cell. The presence of the human genes in every cell of a pig embryo is evidence that the genes went through Select one: a. mRNA transcription b. DNA replication c. mRNA translation d. DNA transcription
b. Neurospora has an arginine amino acid synthesis pathway shown below: ornithine citrulline arginine NH H2N H2N OH H2N HO HO NH2 NH2 ÑH2 Enzyme A Enzyme B Enzyme C ↑ ↑ Gene A Gene B Gene C Suppose I have a neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning. C. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not ornithine supplements. Assming genes A, B and C are unlinked and there is only one mutation per strain: i) What percentage of the progeny will grow on ornithine? ii) What percentage on citrulline? iii) What percentage on arginine? Show your work for i), ii) and iii). |
Row C D. B B. one: A C O phage with radiolabelled protein coat phage with radiolabelled DNA 100 10. phage infects The experiment shown above was designed by bacterium phage infects bacterium EXPERIMENT 1 phage shell is removed EXPERIMENT 2 요 Hershey & Hershey & Chase Meselson & Stahl Meselson & Stahl 8 phage shell is removed 28 no radioactivity in cells ii 48 radioactivity in cells LL and proved that ii DNA replication is semiconservative DNA is the hereditary material DNA replication is semiconservative DNA is the hereditary material (select the row that correctly completes the statement)
This is question is from my micro biology class. 1. OUTLINE the steps that lead to the production of proteins from the genome of a double stranded RNA virus. List any PROCESSES involved and any ENZYMES.
. Susceptibility of the bacteria will be determined by observing for the production of plaques. Describe how these plaques are formed. Would the different strains/species of bacteria be susceptible to bacteriophage T2? Explain why b. How does a viral plaque compares to a bacterial colony? c. Which organism is identified by the use of bacitracin? What does the absence of bacterial growth around the bacitracin disc indicate?
A. Your laboratory requires you to look into the gene identified from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2. The gene has been deposited in NCBI and annotated as STM3777 cytoplasmic protein [ Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ]; GenBank: AE006468.2. 1. Where is this gene location in the bacterial genome? 2. What is the length of the gene? 3. Obtain the FASTA format of this gene sequence. 4. Translate the nucleotide sequence to protein sequence. 5. Can you postulate the function of this gene using bioinformatics tool? Try both nucleotide blast and protein blast.
About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.
I AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I BELIEVED THAT THIS IS " S. PYOGENES. SO I DID A BACITRACIN TEST ON IT. IMAGE 2 SHOWS BACITRACIN TEST IMAGE. ** PLEASE HELP ME IDENTIFY IF THIS IS S. PROGENES AFTER LOOKING AT ALL THE THREE. BACITRACIN SHOULD BE THE CONFIRMATION TEST. IF YOU THINK THAT THIS IS S. PYOGENES AFTER LOOKING AT THE BACITRACIN TEST, THEN PLEAE EXPLAIN HOW DID YOU FIGURE IT OUT FROM THE CONFIRMATION THAT THIS IS S. PYOGENESE FROM BACITRACIN TEST. NEED VALID REASON WHY YOU BELIEVE IT IS S. PYOGENES
describe F plasmid
explain F' plasmid formation
a. You have a test tube containing 5 ml of a solution of bacteriophages, and you would like to estimate the number of bacteriophages in the tube. Assuming the tube actually contains a total of 15 billion bacteriophages, design a serial dilution experiment that would allow you to estimate this number. Ideally, the final plaque-containing plates you count should contain more than 10 and fewer than 1000 plaques.