A microorganism is grown in a fermenter under steady-state conditions. The microorganism growth follows Monod kinetics and the following stoichiometric equation: C3H6O3 +a O2 +b NH3c biomass + d CO2 + e H₂O Given that the biomass yield is 0.415 grams of biomass per gram of substrate, determine the respiratory quotient.

A microorganism is grown in a fermenter under steady-state conditions. The microorganism growth follows Monod kinetics and the following stoichiometric equation: C3H6O3 +a O2 +b NH3c biomass + d CO2 + e H₂O Given that the biomass yield is 0.415 grams of biomass per gram of substrate, determine the respiratory quotient.

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Yeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. = 1.3 g cells / L. The growth mediğim in the reactor, which is well-mixed so the cells have access to all nutrients, contains 150 g CELL DATA ribose (C5H10O5, MW 150), 75 g ammonia (NH3, MW 17), and 85 g oxygen (O2, MW 32). A-10. Determine the maximum concentration of cells, [X], that will form in the bioreactor when the limiting nutrient is consumed. Search Yx/ribose YX/02 YX/NH3 Specific growth rate on limiting nutrient, u Lag phase duration Deceleration phase duration 0.48 g/g 0.87 g/g 0.23 g/g 0.51 h 30 minutes Negligible
The chemical reaction equation for the conversion of ethanol (C2H5OH) to acetic acid (CH3COOH) is: C2H5OH + O2 → CH3COOH + H2OIn a vinegar production industry, acetic acid is produced from ethanol during the growth of the bacterium Acetobacter aceti, whose cellular composition is CH1,8O0,5N0,2. The biomass yield from substrate is 0.14 g/g, while the product yield also from substrate is 0.92 g/g. Ammonia is used as a nitrogen source. Based on this information, answer: (a) What is the oxygen demand for acetic acid production in this crop?(b) What are the cooling requirements of a bioreactor containing 3000 L of ethanol (ρ = 789 kg/m³), to be completely converted into acetic acid?
You have evaluated the rate of aerobic degradation of organic matter in an industrial wastewater and found that it does not follow normal Monod kinetics. After some testing, you have found that the substrate degradation rate follows the following relationship instead: -rut = k0.5XaS0.5 where -rut = substrate utilization rate (mg/L•day) k = 4 (L/mg•d2) S = substrate concentration (mg/L) Xa = active microbial concentration (mg/L) You have also determined coefficients of bacterial growth on this substrate under aerobic conditions to be Y = 0.25 g VSSa/g substrate and b = 0.08/d. (VSSa, active volatile suspended solids) Estimate the effluent substrate concentration when treating the above wastewater in a CSTR (continuously stirred tank reactor) with recycle while operating at a θx (MCRT, mean cell residence time) of 4 day.
Aerobic degradation of an organic compound by mixed cultureof organism in wastewater can be represented by following reaction. C3H6O3 + a O2 + b NH3 → c C5H7NO2 + d H2o + e CO2 A. Determine a, b, c, d and e, if YX/S = 0.4 d X/g S. B. Determine the yield coefficients YX/O2 and YX/NH3. C. Determine the degree of reductions for the substrate, bacteria and RQ for the organisms
The growth of a bacterium follows the Monod model with μmax=0.3 h-1, Ks=2.5 g/l and Yx/s=0.20gx/gs. Calculate the number of continuous mixing tank type bioreactors in series, with 10 L of useful capacity each, necessary for the limiting substrate conversion to be greater than 98%, considering that the system is fed with a flow rate of 2.5 L/ h and the substrate concentration in the feed is 30 g/L.
Under anoxic conditions, biological denitrification of waste water by activated sludge results in the conversion of nitrate to nitrogen gas. Sludge is microorganisms, and these microorganisms carry out this reaction, this is a biological reaction. When acetic acid provides the carbon source, the reaction can be represented as follows: 5CH3COOH + 8NO3 → 4N2+ 10CO2 + 6H20 + 8HO Acetic acid Nitrate a) Is the stoichiometric equation balanced? write down the number of atoms of each species on left side and also on the right side of this equation. b) In the absence of side reactions, what is the yield of nitrogen from acetic acid in g g1? c) A certain waste water contains 6.0 mM acetic acid and 7mM NaNO3. If 25% of the acetic acid and 15% of the nitrate (NO3) are consumed in other reactions (e.g, for growth of the microorganisms in the sludge), which is the limiting substrate in the denitrification reaction? d) For the situation described in part c), what mass of gaseous nitrogen (N2) in…
Write the complete redox reactions for the following microbial metabolisms. Give an example of a type of environment where such bacteria may thrive (will there be plentiful O2? Organics-rich sediment? mineral-bearing rock formations etc?) Oxidation of glucose (C6H12O6) by sulfate (H2SO4) reducers (redox products are CO2 and H2S). Oxidation of ferrous hydroxide (Fe(OH)2) by iron-oxidizing bacteria in oxygen (O2)-rich environment. Redox products are Fe(OH)3 and H2 Please write all redox reactions correctly and explain! Thanks!
It is required to recover active extracellular enzymes from a completed fermentation mediumthat also consists of proteins (<200,000 molecular weight cut-off (MWCO)) and bacterial cells (1-2μm). A cross-flow 0.35 μm microfiltration (MF) with retentate recycled back to the fermenter willbe used over a period of 5 hours during which time flux is assumed to decline linearly. After 5 hours, assuming 95% of the bacteria are retained and given the additionalinformation below, what is the concertation of bacteria remaining in the fermenter and what area,in m2 , of MF membrane is needed? Initial bacterial concentration = 1.2 gdcw /L (dcw = dry cell weight)Volume of medium at the beginning = 15 m3Volume of medium left in the fermenter after 5 hours filtering = 1 m3Initial (starting flux) = 6.5 x 10-5 m sec -1Final flux (after 5 hours of filtering) = 3.9 x 10-5 m sec -
Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.
Lactic acid is produced by using molasses in stirred tank bioreactor . Thermophilic bacteria (45-50 0C) are used during production. Accordingly, how is 1 L of lactic acid produced in this production process? Draw bioreactor and flowchart.
Lactic acid production is carried out in the stirred tank bioreactor. 100 kg molasses entered the production tank initially. Thermophilic bacteria (45-50 0C) are used during production. According to this, a-) Calculate how much lactic acid is produced in this production. b-) draw the bioreactor and its flowchart. (raw material = molasses, the organism used for production = Thermophilic bacteria)
Lactic acid is produced using molasses in a stirred tank bioreactor. Thermophilic bacteria (45-50 0C) are used during production. According to this a-) Calculate how 100 ml of lactic acid is produced in this production process. b-) Calculate how much raw material (molasses) is required for the production of lactic acid in the Stirred tank bioreactor. Draw the bioreactor and flowchart.