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- On the four compounds testes for antibacterial activity using agar well diffusion assay, a. Which compound exhibit antibacterial activity? b. Which of the compounds axhibit bacteriostatic effect ____ and ____.Enzymes MATCHING TYPE a.Erepsin b.Sutilains c.Chymotrypsin d.Urokinase e.Fibrinolysin f.Rennin g.Papain h.Alcalain i.Bromelains j. Pepsin 1.Additive to remove protein stains 2. Relieve symptoms of episiotomy 3. Isolated from human urine or obtained from human kidney cells by tissue culture techniques. 4. Treatment of blood clots within the cardiovascular system, exclusive of thrombi of the coronary and cerebral ateries. 5. Obtained from the stomach of Sus scrofa, Fam. Suidae and has a proenzyme: pepsinogen which is activated by HCl. 6. From Bacillus subtilis, used as wound debridement. 7. Coagulating enzyme that is present in the mucous membrane of the stomach of animals. Important in cheese making. 8. Crystallized from an extract of the pancreatic gland of Ox Bos Taurus used as an ophthalmic solution. 9. Mixture of protein-digesting & milk clotting enzymes from the juice of Ananas comosus used as meat tenderizer. 10. Found in the intestinal juice, Converts proteoses and…1. Why is it necessary to avoid underfilled blood samples in anticoagulant evacuated tubes? 2. Explain the purpose of thixotropic gel in a blood collection tube. 3. What is the rationale behind the sequence of the order of draw? a. Sterile tube (blood culture) b. Blue-top coagulation tube C. Serum tube (glass) with no additive d. Serum tube with clot activator, with or without е. Heparin tube with or without gel plasma separator f. EDTA tube g. Glycolytic inhibitor tube 4. What are the potentially affected tests if an evacuate tube with silica clot activator was filled first before a blue-top coagulation tube?
- 1. cite the differences between tube dilution and microtiter plate dilution. 2. why is it important to isolate the peripheral blood mononuclear cells completely from the other cellular components of the blood? 3. what laboratory assays can be performed to assess the efficiency of the sample preparation and isolation of the PBMC?Give typing answer with explanation and conclusion 8. Considering the principle of the Neutral Red bioassay, describe, in details, the expected appearance of the cheek/buccal cells if exposed to a toxic substance such as cigarette ash or possibly any other type of toxic substance.1. What is an antiserum? 2. What are the potency requirements in an antiserum? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.4 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B
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- Differentiate Streptolysin O from Streptolysin S. What titer is significant for streptococcal infection? Discuss the principle behind ASO titration method based on Neutralization reaction.PLEASE ANSWER BRIEFLY. Thank you. 5. In hairy cell leukemias (HCL), tartrate resistant acid phosphatase is demonstrated. In your opinion, is this the most reliable test to diagnose HCL at present. Explain in not more than 3 sentences. 6. The use of monoclonal antibodies to detect cluster of differentiation (CD) in leukemic cells, mostly immature cells is common in the laboratory. Why do you think this is more valuable than morphological examination of cells in the blood/bone marrow cells.E1. A. Explain how the Snyder agar medium is both a selective and a differential medium. B. How is the differential nature of the medium used to detect susceptibility to dental caries?