5'-GTGTCCGTCTAATATTGTGAGATGTTATATCCCGCCGTCAACACCATCAA-3' --- 3'-CACAGGCAGATTATAACACTCTACAATATAGGGCGGCAGTTGTGGTAGTT-5' 60 80 100 5'-АCAGGATAAТССССТССТGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGСС-3. 3'-TGTCCTATTAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG-5' 1. What are the nucleotides of the resulting MRNA? Indicate the 5' and 3' ends of the MRNA. 2. What are the amino acids translated from the resulting mRNA? 3. Do the underlined nucleotides TAA (indicated in blue) encode a stop codon for the protein? Briefly explain your answer.

Anatomy & Physiology
1st Edition
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Chapter3: The Cellular Level Of Organization
Section: Chapter Questions
Problem 17RQ: Which of the following sequences on a DNA molecule would be complementary to GCTTATAT? TAGGCGCG...
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CHEM 3141: BioChemistry
Quiz no.4 -
The double-stranded DNA sequence for a bacteria is shown below and it's coding for a
hypothetical protein. The strand on top reads 5' to 3' left to right, while the strand on the bottom
reads 5' to 3' right to left. The nucleotides are numbered 1 to 100. REMINDER: For this
problem, transcription begins with and includes the red and underlined C/G (top strand/bottom
strand) base pair and RNA polymerase proceeds from left to right along the DNA.
1.
20
40
5-СТСТСССТСТААТАТТСТGAGAТСТТАТАТСССССССТСААСАССАТСАА-3.
------+----------+
3'-САСAGGCAGATTATAACACTCТАСААТАТАGGGCGGCAGTTCTGGТАСТT-5*
60
80
100
5'-ACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC-3'
-------+
3'-TGTCCTATTAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG-5'
1. What are the nucleotides of the resulting mRNA? Indicate the 5' and 3' ends of the mRNA.
2. What are the amino acids translated from the resulting mRNA?
3. Do the underlined nucleotides TAA (indicated in blue) encode a stop codon for the protein?
Briefly explain your answer.
Transcribed Image Text:1:56 .ll 0.02 KB/S 55 4.pdf CHEM 3141: BioChemistry Quiz no.4 - The double-stranded DNA sequence for a bacteria is shown below and it's coding for a hypothetical protein. The strand on top reads 5' to 3' left to right, while the strand on the bottom reads 5' to 3' right to left. The nucleotides are numbered 1 to 100. REMINDER: For this problem, transcription begins with and includes the red and underlined C/G (top strand/bottom strand) base pair and RNA polymerase proceeds from left to right along the DNA. 1. 20 40 5-СТСТСССТСТААТАТТСТGAGAТСТТАТАТСССССССТСААСАССАТСАА-3. ------+----------+ 3'-САСAGGCAGATTATAACACTCТАСААТАТАGGGCGGCAGTTCTGGТАСТT-5* 60 80 100 5'-ACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC-3' -------+ 3'-TGTCCTATTAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG-5' 1. What are the nucleotides of the resulting mRNA? Indicate the 5' and 3' ends of the mRNA. 2. What are the amino acids translated from the resulting mRNA? 3. Do the underlined nucleotides TAA (indicated in blue) encode a stop codon for the protein? Briefly explain your answer.
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CHEM 2215: BioChemistry
Quiz no.2
1. Identify the acid on the left and its conjugate base on the right in the following equations:
(a) HOCI + H20 + H;O* + OCI
(b) HONH2 + H20 + HONH*+ OH
(c) NH," + H,0 + NH3 + H;0
(d) 2HCO,2+ H,CO; + CO;
(e) PO, + H,PO, + 2HPO,
2. Calculate the pH of water at 25°C and 75°C. The values for pKw at these temperatures are
13.99 and 12.70, respectively.
3. What mass of sodium glycolate (NaC2H;O3) should be added to 400.0 mL of 1.00 M glycolic
acid to produce a buffer solution with a pH of 4.00? K, = 1.47 x 10
4. Tris(hydroxymethyl)aminomethane [(HOCH2);CNH2 Tris, or THAM] is a weak base
frequently used to prepare buffers in biochemistry. Its K, is 1.2 x 10* and pK, is 5.92. The
corresponding pK, is 8.08, which is near the pH of the physiological buffers, and so it exhibits
good buffering capacity at physiological pH. What weight of THAM must be taken with 100 mL
of 0.50 M HCI to prepare 1 L of a pH 7.40 buffer?
5. Calculate the pH of a solution that is 0.200 M in NH; and 0.300 M in NH,CI. Calculate the pH
change that takes place when a 100-mL portion of (A) 0.0500 M NaOH and (B) 0.0500 M HCl is
added to 400 mL of the buffer solution. K, = 1.75 x 105
Transcribed Image Text:1:55 .ll 0.03 KB/S 56 2.pdf CHEM 2215: BioChemistry Quiz no.2 1. Identify the acid on the left and its conjugate base on the right in the following equations: (a) HOCI + H20 + H;O* + OCI (b) HONH2 + H20 + HONH*+ OH (c) NH," + H,0 + NH3 + H;0 (d) 2HCO,2+ H,CO; + CO; (e) PO, + H,PO, + 2HPO, 2. Calculate the pH of water at 25°C and 75°C. The values for pKw at these temperatures are 13.99 and 12.70, respectively. 3. What mass of sodium glycolate (NaC2H;O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? K, = 1.47 x 10 4. Tris(hydroxymethyl)aminomethane [(HOCH2);CNH2 Tris, or THAM] is a weak base frequently used to prepare buffers in biochemistry. Its K, is 1.2 x 10* and pK, is 5.92. The corresponding pK, is 8.08, which is near the pH of the physiological buffers, and so it exhibits good buffering capacity at physiological pH. What weight of THAM must be taken with 100 mL of 0.50 M HCI to prepare 1 L of a pH 7.40 buffer? 5. Calculate the pH of a solution that is 0.200 M in NH; and 0.300 M in NH,CI. Calculate the pH change that takes place when a 100-mL portion of (A) 0.0500 M NaOH and (B) 0.0500 M HCl is added to 400 mL of the buffer solution. K, = 1.75 x 105
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