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- 2. Explain why there is minimal change in the pH when a strong acid is added to a buffer as opposed to when it is added in water.6. Explain how buffer systems are important in organisms. In the human body, bicarbonate and carbonate ions work together to stabilize or buffer the pH of body fluids. What would happen to your blood if these buffering agents were removed? Use the buffers assignment to help you2. Calculate the pH of a buffer containing 0.1 M sodium acetate and 0.2 M acetic acid (pKa = 4.76).
- 1. Describe how you would go about making up the following solutions. a) 150ml GTE buffer (resuspension solution) containing, 20mM Tris pH 8, 5000µM EDTA, 1M Glucose4. Which step consists of purification and buffer exchange?5. A 60-year old woman was admitted to the hospital with a diagnosis of pneumonia. She had beenon a thiazide diuretic for 6 months previously for treatment of heart failure. Her lab results were:K+ 2.1 mMpH 7.64PCO2 32 mm Hg PO2 75 mm Hg HCO3- 33 mMClassify her acid-base disturbance. Hint: graph it out on the pH-HCO3- diagram
- 4. A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water. Volume used from Buffer Final volume, mL original solution, mL 1 5 100 2 10 100 20 100 a. Do they have the same pH? Check the box corresponding your answer. O Yes O No HY 47.1 Basic Biochemistry, Laboratory b. Which is true about their absolute concentrations? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] c. Which is true about their concentration ratios? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] %3! %3D d.…2. You are given 575mL of a .200M solution of NaOH and are adding 600mL of water What is the new concentration?1. Calculate the pH of a buffer solution made from 0.20 M CH3COOH and 0.50 M CH3COO- that has an acid dissociation constant for CH3COOH of 1.8 x 10-5.
- 1. What are the different factors that affects the quality of water? 2. What is the role of a coagulating agent in the process of purification?6. Of the following pairs write which ones could be used as buffers and why: (a) 0.1 M NaOH and 0.1 M NaCH3COO, (b) 0.01 M H2SO4 and 0.01 M K2S04, (c) 0.01 M NH3 and 0.02 M NH4CI, (d) 0.2 M HNO2 and 0.2 M NaNO2.1.Write the equation for the dissociation and re-formation of water
