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- 6. Similar to the class notes (Intro to Genetics), a segment of DNA (shown below) contains a promoter segment (the first 9 base pairs), a ribosome binding segment (the next 6 base pairs), and a segment that codes for protein synthesis which is started by the rest of the base pairs. ACTCCATTGAACCATTTCTATGATCCGCTAACG-... TGAGGTAACTTGGTAAAGATACTAGGCGATTGC-... A. When the DNA is induced to be copied to mRNA, the top strand is coding, meaning that the mRNA makes an identical copy of the lower strand (replacing T with U) The mRNA copy starts with the ribosome binding sequence. What is the sequence of the mRNA that will go to the ribosomes? B. What are the first 6 amino acids of the protein that are coded for by the mRNA? C. What would the amino acid sequence be if... i. a transition mutation occurred on the final G in the mRNA? ii. all of the G & C bases in the protein synthesis portion had transition mutations? iii. a point deletion mutation occurred in the ATA sequence (in the lower strand…2. Which of the following is a true statement concerning condons? Condon specifies one amino acid is found in all eukaryotes, but not in prokaryotes will be read by RNA polymerase during transcription is composed of two nucleotides is comprosed of three amino acids4. Draw and label the Transcription process. 5. Draw and label the Translation process.
- 1. How are nucleotides formed? In details summarize the process of DNA replication. In details summarize the process of Translation and post translation process. In details summarize the process of transcription. Explain how do you sequence the DNA3. In the DNA segment 5'-ATGAGGCATGAGACG-3' (coding strand) 3'-TAСТССGTACTСTGC-5' (template strand) What products would be formed from the segment's replication? Write the mRNA sequence that would be obtained from the segment's transcription. What is the amino acid sequence of the peptide produced from the mRNA?7. Although DNA gets all the glory, it’s actually RNA that does most of the work when cells produce proteins. Explain how RNA is involved in gene expression (i.e., in transcription and translation).
- 1. Determine what amino acid will be formed from the given DNA strand below: 3’ T A C A T G C C G A A T G C C 5’ 2. how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. 3. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used 4. Look at the genetic code to know what amino acid will become part of the polypeptide chain.7( what is the diagram depictin A) transcription and translation return B. DNA replication C -DNA replication transcription and translation D-transcription E- translation 1. Given the piece of MRNA: 5'-CCUGCAGUAUGAAACGCCUGGUAGAAGGUGGGAAGUGGUGCGCC-3' 1. Predict the DNA strand sequence from which it was transcribe 2. List the complementary non-coding DNA Sequence 3. List the Amino acid Sequence of the protein coded for. Use the Genetic Code 2. Below is a short segment of a DNA molecule. Translate the DNA codon into MRNA. 5'- TACCATGAGAATTGTGG TCACCTTTTT-3' 3'- ATGGTACTCTTAACACCAGTGGAAAA A-5' MRNA Sequence Amino Acid Sequence
- 2. Refer to the protein phe-trp-tyr-cys-ala-met-leu, construct a DNA strand that can transcript an mRNA and tRNA that can produced that proteina. mRNA strandb. tRNA strandc. DNA strand (5’-3’)d. DNA strand (3’-5’)5. A molecule with three bases on one end that can bind an amino acid on the other end during gene expression would be which of the following? O transcription factor O TRNA O MRNA O protein O translation factor4. Indicate whether each of the following words orphrases applies to proteins, DNA, or both.a. a macromolecule composed of a string of subunitsb. double-strandedc. four different subunitsd. 20 different subunitse. composed of amino acidsf. composed of nucleotidesg. contains a code used to generate othermacromoleculesh. performs chemical reactions