4. Answer the following questions about dilutions. b You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 mi of saline solution. You take.Imi from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration 6.1X10³ CU ON 61 X 10² CFU 61 X 10² CFU 6.1 X 10² CFU/ You have a culture that has 5x10 cfull of bacteria in it. You performa serial dilution by adding 1 mi of the stock in Pi of TSB in Tube A and mix You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube Band Last you take 1 from tube 8 and add it to a TSA plate. After overnight incubation, you count the colonies you see. How many colonies will you see? 50 Oas You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? Plate B None of the plates can be used Od Plates A. B and C Plate C el Plate A
4. Answer the following questions about dilutions. b You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 mi of saline solution. You take.Imi from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration 6.1X10³ CU ON 61 X 10² CFU 61 X 10² CFU 6.1 X 10² CFU/ You have a culture that has 5x10 cfull of bacteria in it. You performa serial dilution by adding 1 mi of the stock in Pi of TSB in Tube A and mix You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube Band Last you take 1 from tube 8 and add it to a TSA plate. After overnight incubation, you count the colonies you see. How many colonies will you see? 50 Oas You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? Plate B None of the plates can be used Od Plates A. B and C Plate C el Plate A
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:4. Answer the following questions about dilutions.
b.
C
You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of
culture and 9 ml of saline solution. You take.Iml from the most dilute tube
and plate it on a TSA plate. The next day you count 61 colonies. What was
the original concentration
6.1X10³ CFU/
ON 6.1X 10² CFU
Od 61 X 10² CFU/ml
61X10 CFU/
You have a culture that has 5x10 cfu/ml of bacteria in it. You performa
serial dilution by adding 1 mi of the stock in 9ml of TSB inTubeA and mix
You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube Band mix
Last you take 1 ml from tube 8 and add it to a TSA plate. After overnight
incubation, you count the colonies you see. How many colonies will you see?
5000
N 500
Od 50
Oas
Oes
You are doing a series of serial dilutions to find the concentration of bacteria
in CFU/ml in a culture. You plate several of the dilutions tubes and count the
colonies the next day. On plate A you find over 1000 colonies, plate B you
find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used
to determine the concentration in the original tube?
al Plate B
b) None of the plates can be used
Od Plates A. B and C
Plate C
el Plate A
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