21. Which lane shows a digest with Bamtll only? a. I b.I c. II d. IV e. V 22. Which lane shows a digest with EcoRL only? a. I 23. Which lane shows the fragments produced when the plasmid was incubated with both EcoRl and BamH1? a. I b.I c. II d. IV e. V b.I c.II d. IV e. V
21. Which lane shows a digest with Bamtll only? a. I b.I c. II d. IV e. V 22. Which lane shows a digest with EcoRL only? a. I 23. Which lane shows the fragments produced when the plasmid was incubated with both EcoRl and BamH1? a. I b.I c. II d. IV e. V b.I c.II d. IV e. V
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Below is a plasmid with restriction sites for Baml and EcoRI, Several restriction digests were done using these
two enzymes either alone or in combination. Hint: Begin by determining the number and size of the fragments
produced with each enzyme. "kb" stands for kilobases, or thousands of base pairs.
Plasmid
Gel lanes
I | III IV V
6 Kb
Bam HI
Bam HI.
20 Kb
PGEN101
(20 Kb)
2 Kb
11 Kb
Bam HI
8 Kb
8 Kb
4 Kb
6 Kb
Eco RI
3 Kb
21. Which lane shows a digest with BamHl only?
a. I
b.I
c. II
d. IV
e. V
22. Which lane shows a digest with EcoRLonly?
a. I
23. Which lane shows the fragments produced when the plasmid was incubated with both EcoRI and BamH1?
a. I
b.I
c. II
d. IV
e. V
b. I
c. II
d. IV
e. V
Base pairs
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