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- 1. Is the reagent used to cut peptide bonds during amino acid analysis. 2. Is the name of the amino acid present in the active site of carbonic anhydrase. 3. Is the name of the reagent used to poison serine proteases. Is a component of bacterial cell wall other than N-acetylglucosamine? 4. 5. Is the optimal pH of lysozyme.5)A certain aerobic organism is able to metabolize the followingglycolipid CH,OH H H OH HO OH10. Pharmaceutical ingredients are susceptible to microbial attack. Which ONE of the following statements about contamination of a medicine is CORRECT? O Anionic surfactants are generally stable but the alkyl and alkylbenezene sulphonates are readily metabolised. O Humectants, such as sorbitol and glycerol, are readily metabolised when present in a medicine at high concentrations. O Fats and oils are usually attacked when dispersed in non-aqueous formulations. O Sweetening, flavourings and colouring agents are ready substrates for bacteria but in low concentrations are resistant to degradation. O Organic polymers used as thickening agents are subject to microbial polymerisation.
- 1. What will be the properties needed to guarantee survival in a mesophilic condition? Answer this comprehensively and completely.1. Explain how the slope of the line of absorbance vs. time provides information regarding protease activity.We’re going to make our own yogurt using mixed bacteria cultures which contains Lactobacillus casei, Bifidobacterium longum, Lactobacillus bulgaricus, Streptococcus thermophiles, Lactobacillus acidophilus How can you tell if you successfully made yogurt? What are the functions of all different species in the mixed yogurt cultures?
- 1. Determine the minimum inhibitory concentration and the minimum exposure time given the following data: Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) Concentration of ethanol 10 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:1. are organic proteins which can speed up the biochemical reaction that occurs mainly outside or on the microbial cell surface. 2. Clean surface waters are normally saturated with . , which can be removed rabidly in the aerobic oxidation. 3. . and .. are the most importance two measures in water quality required to stabilize the wastewater. 4. The . process are responsible to limits the polluting discharge effect and the recovery time in rivers.The ingredients in the MR/VP Broth are: Peptone (protein) 7g Glucose 5g Phosphate buffer 5g Distilled water 1L Examine the above figure. This figure shows how the pH in the Methyl Red and Voges-Proskauer (MR-VP) broth changes during incubation from time zero to 48 hours with a VP-positive bacteria species. VP-positive bacteria initially produce lactic acid and temporarily lower the pH, but the lowered pH activates the 2,3-butanediol fermentation pathway. Accumulation of its neutral end products such as acetoin and 2,3-butanediol raises the pH by the end of the incubation time. The acetoin that accumulates in the broth also reacts with the reagents added after incubation to produce a red color. If the reagents were added and the results were recorded early, at 24 hours rather than at the full incubation time at 48 hours, would the color result for the VP (+) bacteria be the same at 24 hours than at 48 hours? If different, explain how the color result is different. What chemical…
- 1. Choose one (1) nonprotein nitrogen compound 2. Draw an illustration that shows the generation, metabolism, and excretion of the chosen NPN. 3. The illustration should be as detailed as possible (include how much is being absorbed, excreted, and the like).4. Bacterium Q had the lab results below. What can you tell me about the protein metabolism of this bacterium? Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids and tryptophan? ________________________________________________ Can it desulfur proteins? _______________________________________ Can it produce NH3 from the amino acid phenylalanine?12. Differentiate exudate from transudate in terms of: Exudate Transudate Cause Appearance Specific Gravity Spontaneous Coagulability Protein Content Cells Bacteria