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- True or false? wild type fast plants propagate mostly via self pollination transfected beta-galactosidase requires antibiotics to be expressed properly. the bradford assay allows you to determine the concentration of proteins produced fromtransfected DNAPlease Answer ASAP. Thank u. Genetically modified plants resistant to the herbicide glyphosphate contain a gene from Bacillus thuringiensis. True FalseArial BIUA 11 + .. | I 1 I 3 I 4 i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ Inone +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why? MacBook Air 000
- Pstl. EcoRI Origin of replication (ori) Ampicillin Tetracycline resistance resistance (Amp) (Tet") pBR322 (4,361 bp) BamHI Pvull Sall Recombinant Plasmid DNA Bacterial cell contains... No plasmid DNA pBR322 (no insert) Recombinant plasmid 00,000. Host DNA Transformation of E. coli cells +AMP plate pBR322 Figure 7-5 +TET plate Based on the recombinant plasmid growth pattern (bottom row of blue table), which of the depicted plasmid's restriction sites was used to prepare this sample? Explain how you can tell.I need help. the answer choices for both blanks are diploid, haploid or zaploid.SELECT ALL THAT APPLY. Choose the CORAECT statements from the foilowing Enfuvirtide is a novel antiviral drug that inhibits integrase enzyme Interfering with DNA synthesis by inhibition of the reverse transcriptase enzyme is a strategy used in the treatment of cancer Acyclovir inhibits viral DNA synthesis and is used in the treatment of herpes infections Nevirapine is an antiviral drug that inhibits viral reverse transcriptase enzyme
- Nutritional requirements of white verses red yeast What is the differences in the ability of different strains to grow on different mediums. Propose a hypothesis that helps to explain the presence of red yeast colonies. Why it is more common for cells to be killed by UV light than to become red. Hint: think about how mutagenesis occurs.Yes or no ? No explanation does plasmid dna synthesize in the 5'to3' direction? situ hybridization, northern blot and microarray can assess in situ hybridization of gene expressions in planarians. does luciferase rnai effect planarians development?Multiple Matching. Match the term with its description.___________nucleic acid probe___________nontemplate strand___________template strand___________reverse transcriptase___________Taq polymerase ___________RNAi___________primer___________restriction endonucleasea. enzyme that transcribes RNA into DNAb. complementary strand that blocks mRNA expressionc. the nontranslated strand of DNA or RNAd. enzyme that snips DNA at palindromese. oligonucleotide that initiates the PCRf. strand of nucleic acid that is transcribed or translatedg. thermostable enzyme for synthesizing DNAh. oligonucleotide used in hybridization
- organisms ha varieties. Through this process, several genetically (10) been produced. What I Can Do Activity 7: MATCHY MATCHY Direction: Match the purpose to the components found in the box below. Antibiotic Resistance Gene Multiple Cloning Site Promoter DNA Inserted Gene Sequence Multiple Cloning Site 1. Allows the controlled expression of the desired gene in the presence of an inducing agent (e.g., beta-galactosidase; heat treatment (~65°"C) 2. DNA sequence or portion for the insertion of the desired gene. This section may contain sequences that will be cut by specific restriction endonucleases ( cuts within the molecule) 3. Successful insertion of a gene allows the expression of its protein product. This usually provides a specific trait to the "transformed" bacteria. 4. Provides a way to screen a population of bacteria for those that took up the plasmid. For example, if an ampicillin resistance gene is encoded in the plasmid, then only bacteria 10 t4Plasmid Mapping (what is it? why is it important?).Genome for C. diphtheriae have about 2,500,000 nucleotides, 87% of them are coding. This ingle circular chromosome contains 2,389 genes from which 2,272 proteins are coded. It does not contain any plasmids. The genome contains Pathogenicity Islands (PAIs), which C. diphtheriae has 13. What is a PAI and what are their characteristics?