14. Discuss the significant similarities and differences between Gram-negative and Gram-positive bacteria. Be as thorough and as detailed as possible (compare their width, spacing, vertical and horizontal lattice composition etc). Diagrams are great.
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- 9. Determine if each structure or feature is characteristic of Gram-negative bacteria, Gram-positive bacteria, or both. LPS Teichoic acid Peptidoglycan Nucleoid Outer membrane Thick peptidoglycan cell wall8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. F10 F12 F6 %24 6. R. Home Enter K F G A Shift C Page Up Page Down Alt Ctrl Alt Σ9. Consider the photo below and identify the Gram characteristics as well as the shape/ arrangement of the microbes shown below (example: Gram negative diplococci)
- 11. Complete this diagram with these three species: Escherichia coli, Enterobacter aerogenes, and Staphylococcus aureus. Yes Metallic-green colonies Species: Gram stain: Growth on EMB Thick pink, mucoid colonies Species: Gram stain: No Species: Gram stain: Growth on MSA Yes, with yellow halo No11. Gram-negative bacteria have a thick outer membrane and peptidoglycan layer. True or False.1. Make a table to show the common characteristics used to group bacterial genera or species in the following groups and give one example of genus for each: (a) lactic acid bacteria, (b) coliforms, (c) proteolytic bacteria, (d) psychrotrophic bacteria, and (e) enteric pathogens.
- 14. Other than bacteria list the other 6 types of Microorganisms listed in your textbook.asap1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.3. Why can there be no generalized media that can support all types of microorganisms? Briefly justify in less than 50 words.
- 1. Why is it important to use a small amount of bacteria when making a smear? Give at least 2 reasons. 2. What are the two reasons for “fixing” a smear? 3. Simple staining is useful for determining what three characteristics of a bacterial cell? 4. With respect to bacteria, define morphological arrangement. Explain thoroughly!1. What color does an acid-fast cell stain? 2. Identify two diseases that are caused by acid-fast bacteria. 3. In the endospore stain, what color do the endospores stain? 4. If you performed an endospore stain on Mycobacterium, what color cells would you expect tosee? Why? 5. How do capsules contribute to virulence of an organism? 6. Since you know what lophotrichous and amphitrichous arrangements look like, put thoseterms together to draw an amphilophotrichous bacterium. 7. Streptococcus pneumoniae that are capable of causing pneumonia are encapsulated bacteria(meaning they have a capsule). Describe what you would expect to see under themicroscope after performing a capsule stain with india ink and safranin.Summarize the terms used to characterize microbes based on they physical growth factors including... 1. pH (three terms, pH value, organism example each) 2. Temperature (five terms, temperature ranges, organism example each) 3. Oxygen (five terms, description of growth, organism example each) 4. Salt (three terms, salt concentration, organism example each)