10. What is Relative Centrifugal Field (RCF, in g's) in a centrifuge with a rotor radius of 10 cm spinning at a rotation speed of 12,000 rpm? SOLUTION:
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- SHOW COMPLETE SOLUTION 1. An electrochemical cell consist of 0.2715 Ibs of MnO2, and 0.2524 Ibs of PbO2. A current flow through a 1.1 ohm resistor of electrochemical cell for 45 mins. The reaction takes place at 400k and the total volume of the solution is 504.9 cm^3. Use the standard reduction cell potential table for MnO2 and PbO2: concentration of Mn2+ (2.81 M) and PbO2 (0.95 M) Cell Potential E=0.48 V current when it passes through a 1.1 ohm resistor = 0.44 ampere Determine the following: a. quantity of electricity/charge, Q b. Energy consumed in Joules c. Power in KilowattsA scientist tries to harvest bacterial cells by centrifugation; he used an RPM of 5000. If the radius of the rotor is 144 mm, the relative centrifugal force is 40248 x g 280x 4025 K 402.5 x Question 16 The recipe for making 1 L of LB broth is 10 g Tryptone, 5 g yeast extract, and 10 g sodium chloride. The amount of each substance if you are making 100 mL of LB broth. A Tryptone, 005 yeast extract, and is sodium chloride 0.1 g Tryptone, 0.5 g yeast extract, and 0.1 g sodium chloride 701 Tryptone, 0.05 g yeast extract, and 0.1 g sodium chloride 1 Tryptone 0.5 yeast extract and 1 sodium chladeWhen combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the final concentration of the lysosome solution after adding 8.0 of a biuret reagent to the solution 2.5mg/ml 1mg/ml 0.5mg/ml 5 mg/ml
- 5 900uL of lysozyme solution was added with 100uL of Fraction X into a cuvette. In a kinetic measurement using spectrophotometer, the Abs/min value for lysozyme cell wall hydrolysis activity was -0.040. The settings were at 450nm, 3 second intervals. Calculate the concentration of lysozyme activity that is present, in units of abs/min/mL. If you could show and explain how to calculate, that would be amazingIn cultivation of E.coli strain in a batch stirred and aerated tank, a static gassing out technique method was employed to determine the oxygen transfer coefficient (k̟a) for the bioreactor. After purging the oxygen with nitrogen gas, continuous air supply at air flow rate of 0.1 v.v.m. and impeller speed of about 250 rpm was initiated. Increase in the dissolved oxygen concentration upon the continuous air sparging is recorded by a dissolved oxygen (DO) probe. Using the following data, determine the oxygen transfer coefficient (ka) for the bioreactor. Saturation DO concentration is C*=9 mg/l Time (minute) Cl (mg/l) 1 1 2 3 2.5 4 3 5 4 6.5 5 7.2the optical density of your cell culture is 0.92. How much of this culture should you add to 500 mL of LB to obtain a starting optical density of 0.1?
- Choose all that are correct regarding impeller type choice in bioreactors. O Marine type impellers are ideal for shear sensitive mammalian cells. O Marine type impellers are ideal for less shear sensitive yeast cells. O One function of the impeller is to mix the contents of the bioactor (e.g., nutirents). O None of these options are true. O Impeller type choice depends on the shear sensitivity of the target cells and the solution viscosity. Choose all that are true regarding the phases of cell growth in a bioreactor. O None of these options are true. O There will always be a death phase in the bioreactor, since some cells are dying throughout. O Higher variability in the bioreactor contents is associated with stationary phase. O Higher varaibility in the bioreactor contents is associated with exponential phase when the cells are growing most rapidly. O There will always be a lag phase of growth in a bioreactor since the cells need time to adjust to their new enviroment upon…1. How much 1 M citrate would you need to make 100 mL of 0.5 M citrate? Give the answer in ug/ul 2.If you added 59 grams of Succinate MW=118.1 to 1 L of H2O, what would be the resulting concentrations (in M) of succinate in that solution? 3. What is the volume of a cell that has a diameter of 6 um?Indicate the contrast statements of the following chromatographic techniques of separation. A. A separation of components mixture based on migration rates in a stationary phase by a gaseous or liquid mobile phase. B. The supported materials coated of flat plate or in fibres of paper stationary phase is known as planar chromatography. C. The rate of migration of molecules or analytes is depending on the relative solubility of the solute in the stationary phase and mobile phase. D. Involve an electronic transition of valence shell electron in free atoms to provide analytical information about sample composition.
- If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires 3500xg, what would your setting be in a given centrifuge with a radius of 10cm? Provide computationsYou are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar, you will be preparing the desired concentration from a prepared stock solution. Complete the information in the table and show your computation. Volume of Volume of diluent Desired Stock Solution stock sol'n needed to make Concentration needed 1000mL media 25% 12.5% 5% 1.5%
