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1. Why is NSS ideal for performing the process of "washing" or "elution"? 2. Why is it necessary to wash the red cells with NSS three times prior to their use? 3. What is the importance of preparing a red cell suspension in the laboratory prior to performing the following procedure? 3.1 Blood Typing 3.2 Coomb's Test

1. Why is NSS ideal for performing the process of "washing" or "elution"? 2. Why is it necessary to wash the red cells with NSS three times prior to their use? 3. What is the importance of preparing a red cell suspension in the laboratory prior to performing the following procedure? 3.1 Blood Typing 3.2 Coomb's Test

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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5. Using the following formula, complete the table in preparing red cell suspension with the desired concentration. Show your computations below. % red cell suspension = Amount of washed packed red blood cells Total Volume x 100 Total volume = amount of washed PRBC + amount of NSS to be added Total Volume Amount of washed packed red blood cells 0.10 mL 0.15 mL Solution: 0.20 mL 0.10 mL Desired % 3% 3% 2% 3mL 5mL Amount of NSS to be added 9.80mL
Transcribed Image Text:5. Using the following formula, complete the table in preparing red cell suspension with the desired concentration. Show your computations below. % red cell suspension = Amount of washed packed red blood cells Total Volume x 100 Total volume = amount of washed PRBC + amount of NSS to be added Total Volume Amount of washed packed red blood cells 0.10 mL 0.15 mL Solution: 0.20 mL 0.10 mL Desired % 3% 3% 2% 3mL 5mL Amount of NSS to be added 9.80mL
ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.3 1. Why is NSS ideal for performing the process of "washing" or "elution"? 2. Why is it necessary to wash the red cells with NSS three times prior to their use? 3. What is the importance of preparing a red cell suspension in the laboratory prior to performing the following procedure? 3.1 Blood Typing 3.2 Coomb's Test 4. List the possible sources of error in the preparation of Red Blood Cell suspension?
Transcribed Image Text:ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.3 1. Why is NSS ideal for performing the process of "washing" or "elution"? 2. Why is it necessary to wash the red cells with NSS three times prior to their use? 3. What is the importance of preparing a red cell suspension in the laboratory prior to performing the following procedure? 3.1 Blood Typing 3.2 Coomb's Test 4. List the possible sources of error in the preparation of Red Blood Cell suspension?
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 4. List the possible sources of error in the preparation of Red Blood Cell suspension?

I I I 5. Using the following formula, complete the table in preparing red cell suspension with the desired concentration. Show your computations below. % red cell suspension = Amount of washed packed red blood cells Total Volume x 100 Total volume = amount of washed PRBC + amount of NSS to be added Desired % Amount of washed packed red blood cells 0.10 mL 0.15 mL Solution: 0.20 mL 0.10 mL 3% 3% 2% Total Volume 3mL 5mL Amount of NSS to be added 9.80mL
Transcribed Image Text:I I I 5. Using the following formula, complete the table in preparing red cell suspension with the desired concentration. Show your computations below. % red cell suspension = Amount of washed packed red blood cells Total Volume x 100 Total volume = amount of washed PRBC + amount of NSS to be added Desired % Amount of washed packed red blood cells 0.10 mL 0.15 mL Solution: 0.20 mL 0.10 mL 3% 3% 2% Total Volume 3mL 5mL Amount of NSS to be added 9.80mL
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