1. The chromatography solvent is very polar as it contains alcohol, an acid and water. Based on this information, list all the polar amino acids and arrange them from most polar to least polar.
Q: Which of the alternatives below indicates techniques used both to purify and to concentrate…
A: Proteins are polymers of amino acids linked by peptide bond and it is one of the important…
Q: 4.. After mixing 47 grams of glucose in 5 litres of 1X PBS, the final solution is %. A. 0.0094 B.…
A: Given Values: Weight of glucose = 47 grams The volume of the solution in which 47 grams of glucose…
Q: 3. The following absorbance spectrum was collected. What color is the sample solution? Explain using…
A: As you posted multiple questions, we are supposed to answer only question as per our guidelines.…
Q: 4. What are zwitterions? Do they interfere with the different color reaction tests?
A: The term 'Zwitterion' is originally derived from the German word 'zwitter' which can be roughly…
Q: 2.1 5 (a) Define an acid and a base according to Bronsted-Lowry An acid is: A base is: (b) Explain…
A: Introduction :- Count the hydrogens on each component before and after the reaction to determine if…
Q: 4. What is the importance of pH adjustment in culture media?
A: pH is the measure of the acidity or basicity of a substance, in molecular terms pH is the negative…
Q: 1. The concentration of the glucose stock solution in bottle is 50 µM. This stock solution was…
A: The concentration of the solution was measured by C1V1=C2V2 Whereas, C1 = Concentration of stock…
Q: A pharmacist needs an IV which is 8% (w/v) dextrose in water. How many cc of pure sterile water…
A: Introduction:- We have been given initial volume as 500cc which means 500ml of D10W ( 10%) and we…
Q: 2. What concentration of H (aq) corresponds to each of the following? pH = 10.92 РОН %3D4.32 РОН %3D…
A: pH is the measure of the strength of H+ ion or Hydronium ions in solution. pH at which 50%…
Q: 1. Explain why lipids are insoluble in polar solvents.
A: Introduction:- A lipid is a macro biomolecule or any of various organic compounds that are insoluble…
Q: 4. Match the terms on the left with those on the right: AUG dihydrouridine Esite
A: Above given in the match the following are the terms involved in the translation process studied in…
Q: KOH (aq) is added to a solution of Fe(NO3)3
A: This is the experiment of a precipitation reaction. When two solutions containing soluble salts are…
Q: 0. The process used to join two molecules of nucleic acid together (or any two organic molecules,…
A: Two simple substances are called monomers.
Q: The colligative properties depend on the concentration based on the solvent. True O False
A: The given statement is true . The colligative properties depend on the concentration based on the…
Q: What is the basis for the separation of proteins by the following techniques? (a) gel-filtration…
A: Chromatography is a separation technique of chemical substances into individual components. and the…
Q: 4. Which of the following tests for reducing sugar? a) Iodine b) Benedicts c) Biuret d) Emulsion
A: There is a wide form of sugars in nature and has totally different chemical and biological…
Q: n SDS PAGE the resolving gel : is 4%(w/v) acrylamide,PH )6.8 is 6-20% (w/v) acrylamide…
A: SDS PAGE stands for Sodium Dodecyl Sulphate Poly-Acrylamide Gel Electrophoresis. It is a technique…
Q: 3 C Which gel in SDS-PAGE is used for samples separation?
A: Introduction A laboratory technique called gel electrophoresis is used to separate mixture of DNA,…
Q: If you mix two solution containing Drug X: solution A 100 mL of a 5% w/v solution B 200 mL of a 20%…
A: The resultant concentration will be the sum of two percent concentration. If two solutions are mixed…
Q: 2. Discuss the procedure of Knott's Concentration technique. What are its advantages?
A: Microfilaria: It is a larval stage of the parasitic worm known as the filarial worm. It is…
Q: Why we must used calibrated dropper in preparing digoxin elixir?
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Benedict's solution would test for monosaccharides so how was this used to test sucrose hydrolosis…
A: carbohydrates are organic molecules which are the primary source of energy in living organisms .…
Q: 3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?
A: Sometimes actual culture contains too many generations of microorganisms because of which the colony…
Q: 1. Provide the Principles and detailed procedures of the following tests for the "Color Reaction of…
A: "Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 5…
A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…
Q: UV detection in liquid chromatography requires that the analyte have a
A: HPLC UV detectors are used with high-performance liquid chromatography to detect and identify the…
Q: 3. Identify the IMVIC pattern of the following tubes. For set of tubes that is E. coli positive,…
A: The IMViC tests are a collection of separate assays used in microbiological labs to detect coliform…
Q: 1. Recognize the following types of biomolecules when given images such as the ones below and on the…
A: Introduction Biomolecules are substances that are produced by biological organisms. These are…
Q: 4. Create an illustrated step-by-step schematic diagram of the following Qualitative test for…
A: Lipids are the substances that are soluble in organic solvents like chloroform, and ether and…
Q: 4. What is the total charge of lysine in the strong acidic medium? A) +2 B) -2 C) +1 D) 0 E) -1
A: Proteins are known as the muscle-building substances of the body. They are very complex…
Q: 2. How many milliliters of water would you need to make a 10-2 dilution using 10ml of a culture?
A: Water is then additional to attain the molarity most wanted for particular solution. This process is…
Q: 4. a) The molecule below is a/n: amino acid dipeptide tripeptide (circle one) b) Would the molecule…
A: Amino acids are organic molecules composed of carbon, hydrogen, nitrogen and oxygen. Some of the…
Q: 7) Which one of the below given properties belongs to ceramic based biomaterials ? a) Easier to…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: 3. Briefly explain how you could successfully separate an emulsion between an aqueous solution of…
A: An emulsion is a mixture of two or more liquids that are normally immiscible owing to liquid-liquid…
Q: During the lumbar puncture 3 bottles are used: Why don’t they spin the sample that will be used for…
A:
Q: 3. Prepare 40 ml of a 0.9% agarose gel. Work to assemble a small-sized gel apparatus and pour one…
A: Agarose is a kind of polysaccharide which is obtained from the red seaweed. The agarose is made up…
Q: 1.What is not associated with increased skin cancer a. Arsenic b. Coal tar derivatives c.…
A: Since you have asked multiple questions, we will answer only the first question for you. If you want…
Q: Identify the approximate amount of the visible band II. Describe the trouble shooting problem from…
A: Approximate amount of the visible band is between 50 to 75.
Q: Provide a one-sentence broad generalization regarding the % acrylamide one should use in SDS PAGE…
A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…
Q: Which of the following statements is TRUE about the band of molecules labeled X ( close to where it…
A: Agarose gel electrophoresis is carried out to separate DNA fragments under the influence of an…
Q: o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the…
A: A working solution is different from Stock solution. A Working solution is prepared from stock…
Q: 1. For the test for sulfur in egg albumin, would you conclude that sulfur is present on it? Explain.…
A: Introduction Egg albumin consists of major water with proteins followed by minor amounts of…
Q: 1. Describe the modes of separation in terms of their mobile and stationary phase and/or separation.…
A: Chromatography is a laboratory technique for the separation of a mixture into its components. These…
Q: 9. Añ ester is treated with aqueous acid. What is this reaction called? OA Esterification O B. Acid…
A: Esters are neutral, class of organic compounds that react with water and produces alcohols and…
Q: 1. Which of the following statement/s is/are TRUE for the protein sample?* The sample will give a…
A: Part 1. The Biuret test is used for testing the presence of peptide bonds and can also tell the…
Q: 3. For the tests listed in following table, describe the appearance of a positive result. In the…
A: Benedict’s test is performed by heating the reducing sugar solution with Benedict‘s reagent. The…
Q: drug is order 100mg/m2 po BID. How many milliliters will a patient receive if her BSA is 1.80m2 and…
A: Drug dose calculation is asked in this question. Ordered dose = 100 mg / m2 BSA of patient = 1.80…
1. The chromatography solvent is very polar as it contains alcohol, an acid and water. Based on this information, list all the polar amino acids and arrange them from most polar to least polar.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
- 10. (a) A student performed a thin layer chromatographic separation experiment. In the first run, four standard substances A - azobenzene, B - benzoin, C -cinnamic acid, and D – dextrose were spotted on the plate. The line indicates the solvent front. In the second TLC run a mixture (X) of two of these same substances was separated. Based on Rf values, what is the identity of the unknown mixture X? (b) A second mixture, Y, was run at the same time. What is wrong with the solvent system used to try to separate Y? АвсDAn amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?
- An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.What is the purpose of: A. The concentrated salt solution? B. adding isopropyl alcohol to the solution? Why does it need to be in low temperature?In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 5 cm away from the finish line. The distance travelled from the starting line to finish line is 30 cm. The first amino acid, AA1 travelled 4 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 4 cm ahead of AA2. a) Reproduce the chromatogram. Label. b) Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____
- 1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock?2. How do you prepare 50 mL of a 0.25X solution of Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the stock solution?20. According to the package insert, adding 2.5 mL of sterile water to a 1 g cefazolin vial yields a final volume of 3 mL. What volume of this solution would you need to obtain 200 mg of cefazolin?
- 1. What is the concentration of the solution prepared by diluting 50 µl of ampicillin (1.0mg/ml) to a final volume of 1ml? 2. Determine the volumes of phenol, chloroform, and isoamyl alcohol required to prepare250 ml of a 25:24:1 (v/v) solution.5. RX: Peppermint spirit 2ml Alcohol 10ml Sterile water QS 100ml Approximately how many fluid ounces of sterile water are needed to compound this solution?You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?