1. A Lineweaver-Burk Plot is shown below. 30 25 Curve A y = 3.1207x + 2.4978 20 15 Curve B y = 1.0003x + 2.3602 10 5 -3 1 5 7 11 1/[Catechol] (mM1) With these curves, determine the following enzyme parameters. Show all pertinent solutions. a. Km of Curve A and Curve B b. Vmax of Curve A and Curve B c. Assuming that one of these curves corresponds to the kinetics of one enzyme and one substrate, which curve represents the effect of an inhibitor? Why do you say so? d. What type of inhibition is exhibited by your answer in question c? Why do you say so? 1/V, (units of activity 1)

1. A Lineweaver-Burk Plot is shown below. 30 25 Curve A y = 3.1207x + 2.4978 20 15 Curve B y = 1.0003x + 2.3602 10 5 -3 1 5 7 11 1/[Catechol] (mM1) With these curves, determine the following enzyme parameters. Show all pertinent solutions. a. Km of Curve A and Curve B b. Vmax of Curve A and Curve B c. Assuming that one of these curves corresponds to the kinetics of one enzyme and one substrate, which curve represents the effect of an inhibitor? Why do you say so? d. What type of inhibition is exhibited by your answer in question c? Why do you say so? 1/V, (units of activity 1)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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IR PAP Chemnistr x G diagram show X PAP Chemistry x G What is the be x E PAP Chemistr x E PAP Chemistr x -a Session Timex Mp Rockin a ar-2903012.agilixbuzz.com/student/135113422/activity/c412d902-b23e-4202-b813-76a1f9f4c196 Mastery Assess It 8 que Solis PAP Chemistry-2903012-42100P-1/Le Chatelier's Principle / Lesson 128 All changes saved 8. A chemist tested the reaction of potassium chlorate (KCIO3) and Iron (II) sulfate (FeSo). The Independent varlable is temperature; all other variables are constant. The results were recorded. Temperature (degrees Kelvin) Reaction Rate (grams per second) 285 290 295 These results support which conclusion? O An increase in temperature increassed the mass of each molecule of reaction product. An increase in temperature increased the amount of reactant present. O An increase in temperature increased the catalytic behavior of reactants. O An increase in temperature increased the rate of collision of reactant molecules. PREVIOUS 8 of 25 NEXT SAVE & EXIT…
1. Provided in the Table below kinetic data for an enzymatic reaction that was carried out in the presence (last two columns) and absence (second column = control) of enzyme inhibitor. Both inhibitors were added in each reaction at a concentration of 2 mM. The enzyme concentration was similar in all and was approximately 0.001 ИМ. a. Calculate both Vmax and KM for the control using Lineweaver-Burk curve. b. Provide the type of inhibition for both? Find, KI, for the inhibitor binding to the enzyme, for experiments (2) and (3). d. Calculate the reaction Kcat for the Control in experiment (1). Draw a velocity versus [S] showing Michaelis-Menten curve for the Control. Clearly show Vmax and KM for the enzyme. c. e. [S] (mM) (1) V. [(µmol/(ml.s)] (2) V. (3) V. [(umol/(ml.s)] [(µmol/(ml.s)] 2 7.6 4.4 6.6 4 14.6 8.6 11.4 8. 26.6 16.4 17.8 16 45.8 29.8 24.6 24 60 40.8 28.2
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