. How do you determine the concentration and dilution of a solution using the tube dilution method? 2. Decide on what to do with this scenario. You are required to measure 1 mL of serum to be added to 5 mL reagent in a clinical chemistry assay. However, the amount of specimen that is left for you to work with is only 0.5 mL. What will you do if you are not allowed to take another specimen from the patient anymore?
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1. How do you determine the concentration and dilution of a solution using the tube dilution method?
2. Decide on what to do with this scenario.
You are required to measure 1 mL of serum to be added to 5 mL reagent in a clinical chemistry assay. However, the amount of specimen that is left for you to work with is only 0.5 mL. What will you do if you are not allowed to take another specimen from the patient anymore?
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Solved in 4 steps
- 6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?1. a 1 mL stock sample is added to 9 mL Of diluent to make solution A, and then 2 mL of solution A is added to 18 mL of diluent to make solution B. what is the final dilution with respect to the stock? 2. in most probably number (MPN) testing what media is used for confirming test and what does a positive result looks like? 3. In Most probably number (MPN) testing what can be inferred from a positive result on the presumtive test?
- After centrifugation, the serum had a noticeable red/pink hue. 1. What is the appropriate next action for the medical technologist? 2. How will this affect each parameter to be tested?The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry method
- A nurse reconstituted a vial of 750 mg Cefuroxime Sodium Powder for Injection with 6mL of sterile water for injection. The reconstituted solution was dark amber-colored solution. The package insert states that solution colors range from clear to yellow depending on concentration, diluent, and storage conditions. The nurse was then hesitant to give the patient the solution due to its unusual dark color. Five portions were taken from a batch of cefuroxime sodium. Prior to testing in the instrument, each part was subjected to one of the following conditions: Conditions Specifications Temperature Portion 1: 8°C ± 2°C Portion 2: 30°C ± 2°C Portion 3: 40°C ± 2°C Light Portion 4: Kept in the dark Portion 5: Exposed to direct sunlight 1. Which form of the product – the powder for injection, the reconstituted product, or both – will you subject to the following conditions? Why do you think these forms can best address the problem?Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutes
- The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.1. How do you calculate the initial concentration of a sample? 2. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. ~You add 9 ml of broth (water) to each tube. You add 1 ml of culture to the first tube and mix. You get distracted, and transfer 1ml of the dilutant (mixture of culture and broth) to the third tube instead of the second. You perform the rest of the series as described. High or LOW ?WIDAL TEST Principle: The test depends on the ability of antibody in the patient’s serum to agglutinate the stained bacterial antigens. When this occurs, the aggregates become clearly visible to the naked eye. Materials: Test tube 75 x 12 mm Physiological saline (0.9%) Incubator or water bath Slides Procedure: A. Rapid Slide Test Semi-Quantitative Method 1. Using a graduated pipette add the following amounts of serum to consecutive circles on a slide for each dilution under test. 0.08 ml, 0.04 ml, 0.02 ml, 0.01 ml and 0.005 ml 2. Thoroughly resuspend the antigen and add a drop to the appropriate circle on the slide. 3. Mix the drops and spread to cover the entire test circle. 4. Gently and evenly, rock and rotate the test slide for 1 minute then examine the slide for agglutination. 5. Results obtained correspond to tube agglutination titer of 1:20, 1:40, 1:80, 1:160, 1:320 respectively. 6. It is advisable to confirm a slide…