. For each of the following separation methods covered in class, describe the basics of how that technique works, and what difference in material properties is used to separate molecules by that technique (for example: molecular size/MW, solubility differences, surface charge, ...) ultrafiltration, centrifugation, size-exclusion chromatography, isoelectric focusing. reverse osmosis, adsorption (physisorption), ion exchange, dialysis, affinity chromatography, electrophoresis and

. For each of the following separation methods covered in class, describe the basics of how that technique works, and what difference in material properties is used to separate molecules by that technique (for example: molecular size/MW, solubility differences, surface charge, ...) ultrafiltration, centrifugation, size-exclusion chromatography, isoelectric focusing. reverse osmosis, adsorption (physisorption), ion exchange, dialysis, affinity chromatography, electrophoresis and

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter8: Forms Of Drugs And How They Act

Section: Chapter Questions

Problem 30RQ

See similar textbooks

Similar questions

Which of the following correctly matches the chromatography technique with the molecular property being exploited for separation? ion-exchange: charge,affinity: polarity, gel-filtration: molecular mass ion-exchange: charge, affinity:molecular binding preference,gel-filtration: molecular size ion-exchange: charge,affinity: molecular binding preference,gel-filtration: isoilectric point ion-exchange: ion binding preference,affinity: molecular size,gel-filtration:polarity
1. Describe the modes of separation in terms of their mobile and stationary phase and/or separation. Mobile phase Stationary phase Modes of Separation Normal-phase chromatography nonpolar Reversed-phase chromatography polar polar nonpolar Ion-exchange chromatography Size-exclusion chromatography Affinity chromatography Analytes/Separation
There are two proteins in a pH 7 buffer, what you know is: one has a lot of Asp and Glu, the other one has a lot of Lys, Arg and His. Below which approach will get the two proteins separated? gel filtration chromatography ultracentrifugation salting out ion exchange chromatography affinity chromatography
Explain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
PART V: PRECIPITATION BY ALKALINE REAGENTS RESULT and OBSERVATION TUBE NO. 1. 3 mL of TREATMENT 2 mL of NaOH solution egg albumin solution QUESTION: Describe the protein solution in each of the tubes after the addition of alkaloidal reagents Generalization:
Joshua is showing a bacterium having high pH requirement for its growth. With this, he has to prepare a minimal medium at pH 9. Adding 0.5 ml of the pH indicator to 10 ml of the medium, the solution remained colorless. The pH of the medium was adjusted using 2.25 ml of the 0.05 N titrant. c.1. What was the pH indicator used by Joshua? c.2. What was the titrant he used?
ANSWER TO THE FOLLOWING QUESTION IN DETAIL What is the basic principle of GPC (gel permeation chromatography)?
Gel-filtration chromatography separates molecules according to their size . Smaller molecules diffuse faster in solution than larger ones, yet smaller molecules migrate more slowly through a gel- filtration column than larger ones. explain this paradox. What should happen at very rapid flow rates?
POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What is your observation from potato A to D? 2. What can you conclude by comparing potato A and potato B? 3. What can you conclude by comparing potato A and potato C?
Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: 1:10 Lab diluted Lab Tincture bleach Disinfectant Isopropanol soap Lysol of iodine BL DIS ISP SO LYS T of I E.coli 40 mm 20 mm 10 mm 5mm 15mm 60 mm Bacillus 50 mm 35 mm 15 mm 8mm 20mm 70 mm cereus chemical (s) best at inhibiting both Gram- negative and Gram-positive bacteria [Choose ] [Choose] chemical least effective at inhibiting both Soap bacteria 1:10 freshly diluted bleach chemical recommended by CDC for decontamination of surfaces contaminate with blod possibly carrying HIV/Hepatitis B or C viruses Tincture of lodine
POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What can you conclude by comparing potato A and potato D? 2. The liquid in the cavity of some potatoes came from where? 3. What is your conclusion about the experiment?
Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: 1:10 Lab diluted Lab Tincture bleach Disinfectant Isopropanol soap Lysol of iodine BL DIS ISP SO LYS T of I E.coli 40 mm 20 mm 10mm 5mm 15mm 60 mm Bacillus 50 mm 35 mm 15 mm 8mm 20mm 70 mm cereus chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical least effective at inhibiting both [ Choose ] bacteria