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12/26/23, 3:26 PM
12.1 PCR Review Questions: 23F-LIFESCI-7A-LEC-1 Cell and Molecular Biology (cross-listed)
https://bruinlearn.ucla.edu/courses/170946/quizzes/983732?module_item_id=6211396
1/4
12.1 PCR Review Questions Due
Nov 30 at 2pm
Points
5
Questions
5
Time Limit
None
Allowed Attempts
2
Attempt History
Attempt
Time
Score
KEPT
Attempt 2
2 minutes
5 out of 5
LATEST
Attempt 2
2 minutes
5 out of 5
Attempt 1
2 minutes
4 out of 5
Score for this attempt: 5
out of 5
Submitted Nov 30 at 1:47am
This attempt took 2 minutes.
1 / 1 pts
Question 1
Select the correct order of steps during RCR.
denaturation, annealing, extension Correct!
Correct!
annealing, initiation, termination denaturation, extension, termination initiation, extension, termination initiation, denaturation, extension 1 / 1 pts
Question 2
12/26/23, 3:26 PM
12.1 PCR Review Questions: 23F-LIFESCI-7A-LEC-1 Cell and Molecular Biology (cross-listed)
https://bruinlearn.ucla.edu/courses/170946/quizzes/983732?module_item_id=6211396
2/4
What is the benefit of using Taq
polymerase in PCR?
Because it is taken from bacteria that live in high temperatures, it stays
active during denaturation steps of the reaction.
Correct!
Correct!
Because it is taken from bacteria, this enzyme works much more efficiently
than other types of DNA polymerase.
Because it is taken from bacteria that live in high temperatures, it does not
have a proofreading function.
None of the other answer options is correct. Because it is taken from bacteria, it makes fewer mistakes. 1 / 1 pts
Question 3
The polymerase chain reaction (PCR) is used to generate:
a single copy of a targeted region of DNA. multiple copies of a targeted region of DNA. Correct!
Correct!
single copies of whole chromosomes. multiple copies of whole chromosomes and a targeted region of DNA. multiple copies of whole chromosomes.
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ersonal/eenongen_my_tnstate_edu/_layouts/15/doc.aspx?sourcedoc={a6b083c9-a226-4c31... ☆
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Picture
Editing
A
В
...
During nucleic acid hybridization, the probe is labelled
Question 1 options:
for DNA stability
to increase probe-test DNA binding
to identify the location of probe and the test DNA
binding
for amplification
Question 2
6.
9.
10
IV
13
14
Which of the following best describes the trait in the pedigree?
Question 2 options:
X-linked dominant
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autosomal domiant
autosomal recessive
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rences
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Editing v
A^
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Question 1
During nucleic acid hybridization, the probe is labelled
for DNA stability
to increase probe-test DNA binding
to identify the location of probe and the test DNA
binding
for amplification
Question 2
10
IV
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Which of the following best describes the trait in the pedigree?
Question 2 options:
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Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4
Polymerase Chain Reaction
Questions:
1. What are the materials used for the polymerase chain reaction?
2. Draw a schematic diagram of the procedure in PCR.
3. Why is it important to design the primers at the start of the laboratory procedure?
4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer?
5. What is the use for magnesium chloride?
6. How much template DNA is added? What is the concentration of the primers?
7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature?
8. In this particular PCR experiment, how many cycles was used?
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Question:-
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The father has the following genotype: A1, A2, B24, B10, Cw4 and Cw7 and the mother is A1, A1, B11, B7, Cw5 and Cw8. Their first boy is A1, A1, B24, B11, Cw7 and Cw8. What is the probability that the second child is compatible with his/her brother?
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וןווד
←
Q
Lab Report 6 worksheets 314 F22 .DOCX
File Edit View Insert Format Tools Help
A 100%
¿
Summary
Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X
https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit
Outline
Headings you add to the document will
appear here.
Normal text
Times ...
12 + B I U
2
18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the
restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme
BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme
individually and then in combination, and the resulting fragment sizes are determined by
means of electrophoresis. The results are as follows:
1
Restriction Enzyme(s)
EcoRI
BamHI
HindIII
EcoRI and BamHI
EcoRI and HindIII
BamHI and HindIII
3
Practice
====•=•€ EX
Fragment lengths (base pairs)
430 bp, 375 bp
470 bp, 335 bp
Lab Report 6…
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BIOTECHNOLGY
Date:
Name:
Instructor:
Section/Group:.
POST-LAB QUESTIONS
1. In one or two sentences, summarize the technique of gel electrophoresis.
2. How does the process of gel electrophoresis separate DNA fragments?
3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process?
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RNA
2. The Continuity of life is based on heritable information in the form of DNA, and structure
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3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell
growth. The recombinant protein can be considered a product of cell culture even though it is not
secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as
the nitrogen source for aerobic respiration of glucose.
The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excluding
recombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein from
glucose is about 20% of that for cells.
How much ammonia is required?
What is the oxygen demand?
If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygen
requirements for wild-type E. coli that is unable to synthesize recombinant protein?
а.
b.
с.
24°C
08:39
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27/04/2022
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Correct:
The N1303K mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303.
Correct:
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Indirect ELISA can detect polygenic gene expression.
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The objective is to interpret the results of an RNA-Seq analysis to identify differentially expressed genes in breast cancer using figure 1. The data provided includes gene symbols, chromosome location, start and end points, strand, fold change, log2 fold change, p-value, and false discovery rate (FDR). The RNA-Seq analysis has identified several genes that are differentially expressed in breast cancer. These genes are located on various chromosomes and have varying levels of fold change, indicating the degree to which their expression levels differ between normal and cancerous cells. The gene with the highest fold change is EYA4, located on chromosome 6, with a fold change of 3604.4176. This indicates that the expression of this gene is over 3600 times higher in cancer cells compared to normal cells. The log2 fold change is 11.81555, which is a measure of the magnitude of the difference in gene expression. The p-value for this gene is extremely low…
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Question 23
Match the following events in the recombinant DNA and cloning process
Prompts
gene of interest and the DNA vector are separately cleaved to
generate ends that can join
DNA is introduced to host cells
vector DNA and the gene of interest are joined together
only the clones with the recombinant DNA are used for
downstream applications
Submitted Answers
Choose a match
transformation
marker-assisted selection
directional cloning
ligation by a ligase action
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c. How do you think these applications will change the world in the future?
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Module 10 DNA & Protein - 20
O Module 9 Genetics - 2021SP/B X
B Take Test: Heredity 2 Homewor X
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* Question Completion Status:
QUESTION 1
Some dogs bark while following a scent trail; others are silent. The barker allele is dominant (B) to the silent recessive allele (b). The allele for straight tails
is dominant (S) over the allele for curly tails (s). If two dogs are heterozygous for both traits, which of these correctly identifies the genotypes of the
parents?
O a. BbSs x BbSs
O b. BbSs x bbss
O c. BbSs x bbSs
O d. Bbss x bbSs
QUESTION 2
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URGENT
Conjugate base questions
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Complete the sentences. Each term may be used more than once.
In a circular bacterial chromosome, the structure of DNA is a
If DNA is twisted in the
Overwinding results in
If DNA is twisted in the
Underwinding results in
One effect of
double helix.
direction, it becomes overwound.
supercoiling.
direction, it becomes underwound.
supercoiling.
double helix.
Answer Bank
positive
negative
right-handed
left-handed
supercoiling in bacterial chromosomes is to promote separation of the two strands of DNA in the
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134
LABORATORY MANUAL IN HUMAN HISTOLOGY
ells U23T RAIUDBAL
Size of the Cells
Shape of the Nucleus
Degree of Chromatin
Condensation
Presence or Absence of
Nucleoli
CE 1215) 1-XXI
Blood Cells
Cytoplasmic Staining
Presence of Cytoplasmic
Granules
Bone Marrow Cell
29
3000 va
010
Na
Y
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The variation in the length of tandem repeat of microsatellite DNA has serious translational affects as this is due to its coding region.
Correct:
If one parent has sickle cell anemia and other has carrier genotype than there is 25 % chance that any offspring is carrier.
Correct:
Sickled WBC block the flow of blood and Calcium as they stick together and caused by frame shift mutation.
Correct:
The N1303K mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303.
Correct:
If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates Blood type B.
Correct:
Indirect ELISA can detect polygenic gene expression.
Correct:
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what is the frequency of the rarest variant you can detect during deep sequencing of a tumor cell if you coverage is 250X?
- 0.5%
- 0.4%
- 25%
- 4%
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Question 6:
The largest chromosome in Mischievous gremlinus has a length of 9 x 107 base pairs. The
rate of DNA synthesis is 3,000 bp per minute at each replication fork.
a. How long does it take to replicate the entire chromosome from a single origin of
replication located exactly in the middle of the chromosome? Assume no pauses.
I
b. Experiments performed by Professor Trink reveal that it takes only 20 minutes for
a growing cell to replicate this chromosome. What is the minimum number of
replicons present on the chromosome?
Florian Fischer
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Editing
153
23
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Lesson 2
Focus Questions
1. What chemicals and molecules are needed for PCR, and what is the function of each
component?
2. Examine the 150 base promoter sequence below.
Kaylee Kauff
5'TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG
GTATCATTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC
ACCCACGAGG AGC ATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA3'
Write in the sequence of the complementary strand and mark the 3' and 5' ends of the
complementary strand.
43
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