Why is storing the spleens on Dulbecco’s phosphate-buffered saline solution containing 3% fetal bovine serum and 1% antibiotic-antimycotic on ice done in splenocyte preparation?
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Why is storing the spleens on Dulbecco’s phosphate-buffered saline solution containing 3% fetal bovine serum and 1% antibiotic-antimycotic on ice done in splenocyte preparation?
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- A 3-year-old child has a history of serious infections and is currently hospitalized with meningitis. The doctor suspects that he may have a complement deficiency and orders testing. A buffer that chelates calcium was added to specimen. There was a decrease radial haemolysis on agarose plate a) Which complement pathway accounted for the result obtained and how will the result be reported in terms of hemolysis at 50 %? b) What is hydrodynamic focusing and why is it an important feature of an instrument.Define α (alpha) hemolytic, β (beta) hemolytic, and γ (gamma) hemolytic and describe how each look on blood agar platesWhat is the purpose of an agglutination reaction test?
- Name the cells in the islets of langerhans which make glucagon and summarize the principles and procedures specific to how these cells were identified in immunostaining. word limit 250Describe the method, time frame, and rationale for the tuberculinskin test. What component of the immune response does thistest detect?A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?
- 7 mL of a 1:50 dilution is needed to run a specific serological test. How much serum and how much diluent are needed to make this dilution? A 1:5 dilution of patient serum is necessary to run a serological test. There is 0.2 mL of serum that can be used. What amount of diluent is necessary to make this dilution using all of the serum?Mangaba, the laboratory technician, is interested in determining narcotics in saliva at this concentrations > 10 mg/L (μg/mL). One approach being considered is to use an immunochemical assay. Which immunoassay would be most suitable for the desired assay? Explain. Describe the immunoassays that are sensitive enough to measure as little as 10 mg/L of antigen? Which of these assays can quantitate urine albumin? Which of these assays are technically complex or require dedicated, expensive instrumentation?When a patient exhibits a four-fold or greater rise in antibody titer against a specific microbial antigen between acute-phase and convalescent-phase serum samples, this is considered to be a “seroconversion”. Which of the following would be a correct interpretation of a seroconversion? what option below is the answer? None of the other four answers are a correct interpretation of a seroconversion These results show that the microbe itself has been detected in the patient’s sample(s) These findings cannot be interpreted because antibodies aren’t specific enough for antigens There is no evidence of current active infection because that would require that the antibody titer is decreasing rather than increasing The results show evidence of current or recent infection by the specific microbe because there has been an active immunologic response to its antigen
- Why is washing with Hank’s balanced salt solution with 0.5% antibiotic-antimycotic done for Splenocyte Preparation?How does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines)A 155-pound patient (height 5'9") is ordered interferon Alfa-2b IM 2 million 3 times a week. The drug is available 10 MU in 1 mL. How many mL will the patient need per week?