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- Why is this technique used?Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?What is the genus and species of your bacteria? And What led you to confirm this result and why? Please discuss. ☆My bacteria is Alcaligenes faecalis
- In STR typing, the homozygous condition will create a higher peak than the heterozygous condition in terms of relative fluorescence. How do the capillary electrophoresis and the electropherogram correlate?You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions: a) Which technique will you use to isolate mitochondria? b) What happens to the pH of the medium when the medium is kept anaerobic? c) What happens when O2-saturated saline is added to the mixture?What is density-gradient centrifugation? What are its advantages and disadvantages in subcellular fractionation?
- Draw a dilution scheme for a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.How will you know if the bacterial cells were transformed during the lab? notes are attached belowIn bealdle and tatums experiments the immediate effect of the X-ray treatments was ?