tube 1: 5ml milk, 10 drops phenol red, 5 ml distilled water tube 2: 5ml milk, 10 drops phenol red, 5 ml distilled water, bile salts * why are 5ml of water added to tubes 1 & 2?
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Question:-
tube 1: 5ml milk, 10 drops phenol red, 5 ml distilled water
tube 2: 5ml milk, 10 drops phenol red, 5 ml distilled water, bile salts
* why are 5ml of water added to tubes 1 & 2?
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- Total amount of yogurt in the container: 170gram The actual amount of yogurt added to Tube 1 is 1 gram. The actual volume in Tube 1 is 10ml. Please help with number 4 and 5. Thanks!Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.Immediately after stirring 1 hour after stiring Original misture Suction filiration Figure 1 Figure 2 Figure 3 Various tests are performed on the mixtures as shown in the figures above. Identify any correct explanations of these tests below. [Check all statements that are true.] In figure 1, a laser is passed through two tubes of clear liquid. The liquid in the tube on the left is a solution, while the liquid in the right tube is not a solution. O In figure 2, a recently stirred mixture is allowed to stand for one hour. The original mixture is a solution while the mixture after 1 hour is not a solution. In figure 3. a mixture is filtered using suction filtration. A solid remains on the filter. The original mixture was a solution.
- Is there any other way to do this excersise? Aparently the correct answer is 1.32 x10^-4 s^-1Desired Desired Initial Volume of Stock or Volume of Concentration Volume Concentration Previous Dilution Distilled Water (C2) (V2) (C1) (V1), µL (V2- V1), µL 100 mg/dL 1.2 mL 200 mg/dL 75 mg/dL 1.2 mL 100 mg/dL* 50 mg/dL 1.2 mL 25 mg/dL 1.2 mLA 55-year-old female patient who is positive for MRSA is having laparoscopic cholecystectomy. During the procedure, the surgeon encounters unexpected bleeding from the liver bed and decides to convert to an open cholecystectomy. The surgeon requests half-strength diatrizoate sodium solution. You have 30mL of diatrizoate sodium on the sterile field and a 50mL syringe. How much diatrizoate sodium and how much normal Celine are added to the syringe to prepare the medication as requested by the surgeon?
- What does the phrase "pipetting up and down" mean and why is this technique used? The phrase "pipetting up & down" means to triturate. Th technique is used to mix (or homogenize) a solutic 7 On what part of a microcentrifuge tube should you write a label? Describe the order in which you filled the tubes in Step 2 of Procedure 4. Did this order result in maximum efficiency? If not, what order would be most efficient? 9 What does the phrase "spinning down" mean and why is this technique used? The word 'spinning down" means, To diminish in energy i to slow down o out; to be gradually ended or concelled. It is used to reduce its spin speed from that required for reading and writing. 10 Why was the comb placed in the middle rather than at one end of the gel for this electrophoresis experime- The comb is in the center of the gel Since the dyes used have positive or negative charges and can therefore migrate in differenLabel three sets of eight microfuge tubes (1-8) and add the volumes of BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Be sure to calculate, and enter, the values for column 4 of this table. Table 3 STD Curve Volume BSA Volume Water (µl) Final BSA Concentration tube (mg/ml) Stock (ul) number 1 100 2 95 3 10 90 4 20 80 5 40 60 6 60 40 7 80 20 100 00An IV bag contains 250 mL of a 5% mannitol solution. How many grams of mannitol are in the IV bag?
- Prepare the following using ingredients from your kitchen: Egg white from one egg Full cream milk (about 10 tbsp) Vinegar Clear bottles, 2 Spoons for stirring Heating pans Muslin cloth or thin handkerchief 6. 1. Add the egg white to one of the clear bottles and the full cream milk (do not use reconstituted or evaporated milk) to the other. Add vinegar to both, one teaspoon-full at a time until about 5 teaspoon-full has been added. Add more up to a maximum of 2x the original. What do you observe? 2. Allow the solution to pass through your muslin cloth or thin handkerchief. 3. Transfer the egg white-vinegar mixture to a heating pan, and the milk- vinegar to another heating pan. Heat both up to almost boiling. What do you observe? 4. Filter both solutions again using the muslin cloth or thin handkerchief. Record your observations. Questions: 1. What is the major protein found in egg white? In milk? 2. What caused the results you observed? What is/are responsible for these? 3. What would…Give one or two product of Milk and answer the following queston: look at the labels of the different milk preparations: 1. Method of sterilization used. 2. What is the Bacterial culture and flavor used if there is any. 3. What is the Fat, Protein & Carbohydrate(sugar) content? 4. Is fortification done? What substances has been fortified (based on the label)? 5. Are Vitamins & amino acids added? Name them based on the label.Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…