The template (antisense) strands of two complete (double-stranded) DNA molecules have the base sequences shown in the table below. Two replicationexperiments are done with each molecule:1. In Experiment #1, samples of each DNA molecule are incubated with radioactive adenine, along with appropriate replication enzymes, ATP, adenine,thymine, and cytosine.Experiment #1 is stopped when each DNA molecule has replicated once.2. In Experiment #2, all the DNA molecules from #1 are purified, and then incubated with with a similar reaction mixture - except non-radioactive adenineis used this time.Experiment #2 is stopped when each DNA molecule has replicated one more time.Predict the percentage of DNA in each sample that is radioactive after each experiment. Round your answers to the nearest percent.DNAtemplate strand sequence3'- T C C C T A -5'3'- G G C C G C -5'% of DNA radioactive after ...Experiment #1Experiment #2%%%%XŚ

Semiconservative replicationMatthew Meselson and Franklin Stahl provided the experiment which shows…

Answered: The template (antisense) strands of two…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Match the following descriptions with the enzymes involved in DNA replication. 1. Adds an RNA primer to begin elongation 2. Removes the RNA primer from the beginning of the newly constructed strands 3. Splices lagging strand segments 4. Cleaves the rung of the DNA double helix ladder Description: DNA DNA Helicase Primase Enzyme: Polymerase Ligase
There are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).
K The template (antisense) strands of two complete (double-stranded) DNA molecules have the base sequences shown in the table below. Two replication experiments are done with each molecule: 1. In Experiment #1, samples of each DNA molecule are incubated with radioactive cytosine, along with appropriate replication enzymes, ATP, adenine, thymine, and cytosine. Experiment #1 is stopped when each DNA molecule has replicated once. 2. In Experiment #2, all the DNA molecules from #1 are purified, and then incubated with with a similar reaction mixture - except non-radioactive cytosine is used this time. Experiment #2 is stopped when each DNA molecule has replicated one more time. Predict the percentage of DNA in each sample that is radioactive after each experiment. Round your answers to the nearest percent. DNA template strand sequence 3'-GAAGCC -5' 3'-ACCTTA-5' Explanation Check - % of DNA radioactive after... Experiment #2 Experiment #1 0% % % % Q Search X S © 2023 McGraw Hill LLC. All…
An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double- stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation? DNA Ligase DNA polymerase lII primase DNA gyrase Single-stranded DNA-binding protein (SSB)
The steps listed below are necessary in order to of copy double-stranded DNA. Place them in order from first to last. Note that all or only a some steps may be used. 1: Synthesis is initiated on the 3-hydroxyl group of the primer. 2: Two strands of a duplex DNA molecule are separated. 3: DNA polymerase makes a DNA chain using an existing chain as a template. 4: A primer base-pairs with the complementary region of the DNA to be copied. 5: A primer base-pairs with the okazaki fragment of the DNA to be copied. 1,2,3,4 4,1,2,5, 3 2,4,1,3 2,4,3,1 3,4,1,2 2,4,3,5,1
What proteins are crucial for creating and maintaining DNA replication forks? Choose the best explanation. Question 2 options: Helicase creates the replication fork; primase keeps the single strands from closing shut. Helicase creates the replication fork; single-strand binding proteins keep the single strands from reuniting. Ligase creates the replication fork; DNA polymerase II keeps the single strands from reuniting. Helicase creates the replication fork; ligase keeps the single strands from closing shut.
Which of the following are differences between prokaryotic DNA replication and eukaryotic DNA replication? (select all that apply) Group of answer choices: 1. Eukaryotic DNA is linear, while prokaryotic DNA is circular and undergoes theta replication 2. Eukaryotic genomes often have multiple chromosomes, while prokaryotic genomes are usually only made up of one chromosome 3. DNA polymerase 3 adds dNTPs to the growing DNA strand in eukaryotes, while DNA polymerase 2 adds dNTPs to the growing DNA strand in prokaryotes 4. In eukaryotes, DNA polymerase requires a primer to add nucleotides to growing DNA strand, while in prokaryotes, no primer is needed to add nucleotides to the growing DNA strand 5. Longer telomeres are added to the end of prokaryotic chromosomes than are added to the ends of eukaryotic chromosomes 6. Eukaryotic DNA will have multiple origins of replication, while prokaryotic DNA only have one
The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA Newly synthesized strand pocoar Leading-strand template A New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saaragon - Next Okazaki fragment will start here Parental DNA helix
In one, simple sentence define the function of the following 1. Helicase = 2. Alpha subunit of DNA polymerase III =
Below is a picture of a single origin of replication in a eukaryotic cell. 5' 3' 5' 1. On the figure above, Draw out where the following molecules will be located: Helicase; Sliding Clamp, Single Strand Binding Protein. 2. On the right hand side of the dotted line, the replication of which template strand (top or bottom) will be continuous by DNA polymerase? 3. On the left hand side of the dotted line, the complete replication of which template strand (top or bottom) will be more affected by a mutation that causes DNA ligase to be partially functional?
Please match the following enzymes with their correct functions. Primase, Gyrase, Ligase, Polymerase, Helicase, Topoisomerase 1.Unwinds DNA at the replication fork. 2.Makes and reseal breaks in the double-helical DNA to release the torque that builds up as a result of unwinding at the replication fork. 3.Synthesizes a short RNA to provide a 3’ -OH group for the attachment of DNA nucleotides. 4.Removed RNA primers and replace them with DNA. 5.Joins Okazaki fragments.
In the following diagram of a replication fork, primers are shown as thick black lines and newly synthesized DNA is shown as squiggly lines. Enzymes are shown as circles or boxes. Click on DNA polymerase I 3' 5' 7 c 34 % G Search or type URL 3' MacBook Pro & 5' + (: * II in 3 5' 3' +

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