How does one measure Km using a Lineweaver-Burk plot? 1. By determining the reciprocal of the y-intercept 2. By determining the negative reciprocal of the x-intercept 3. By determining the slope and multiplying it by kcat 4. By determining the saturation point
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How does one measure Km using a Lineweaver-Burk plot?
1. By determining the reciprocal of the y-intercept
2. By determining the negative reciprocal of the x-intercept
3. By determining the slope and multiplying it by kcat
4. By determining the saturation point
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- For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.what are the complications or the low-reliability in determining the value of KM using graphical methods from a Michaelis-Menten Plotin basic spectrophotometer technique, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 CM-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2.
- From the basic spectrophotometer technique, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of light the beam transmitted through a 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 25mg/mL. Compare between 1 and 2.After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2
- - Why Michalis plot is not used, instead double reciprocal plot is used to determine KM and Vmax?Create one bar graph summarizing both the Tewameter and Corneometer readings (mention this graph in the text as Figure 1) Give a short description of Figure 1 as figure title (Figure 1) Label both axes On the graph, plot the average numbers and standard deviation, not the individual measurements Standard deviation should be displayed on the top of the average bars as an error barFor the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.
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