Fill-in 1. A___ is a glass or plastic rectangle on which specimens are mounted. 2. A _?__ is at the base of the body tube, allowing the selection of different objective lenses. 3. Below the stage is a high-intensity _?__ or other light source. 4. Different lens sets, or _?__, are attached to the revolving nosepiece. 5. If the stage has a bracket that moves the slide, it is called a(n) __?___stage. 6. The allows the user to limit the amount of light from a steady light source. 7. The set of lenses closest to the viewer's eye is the _?__ 8. The is a lens under the stage that concentrates light.
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- Preparing a wet mount of the letter "e”. 1. Obtain a prepared ‘e’ slide. 2. Focus the slide first with the scanning objective, then click to lower power and focus again. 3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fine adjustment knob. 4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification. The LPO, HPO, and Oil ImmersionPreparing a wet mount of the letter "e”.1. Obtain a prepared ‘e’ slide.2. Focus the slide first with the scanning objective, then click to lower power and focus again.3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fineadjustment knob.4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification of Oil Immersion objective1. After focusing to LPO, you were asked by your teacher to shift to HPO (nigh power objective) but the view of the specimen is blurred. What part are you going to manipulate for the specimen to have a sharper focus? A. Mirror B. Fine adjustment knob D. Inclination joint C. Stage clips 2. Forceps are used to hold and fix the specimen on the glass slide. What household material can be used if you don't have any forceps? A. Fork B. Tweezer C. Spoon 3. The formula of total magnification (MT) is the product of magnification of the eyepiece (ME) and magnification of the objective lenses (Mo) like LPO, MPO, or HPO. Which equation represents the statement? A. MT = ME(Mo) C. MT = ME + Mo 4 Compute for the total magnification of an onion skin if the eyepiece has 4X magnification and the LPO has a magnification of 10X. А. 40X C. 2.5X D. Tong B. MT = ME- Mo D. MT = ME/Mo В. 14X D. 6X 5. Solve for the magnification of the eyepiece if the total magnification is 150X and the LPO has a magnification…
- Contrast for a specimen may be adjusted by _____. Question options: Adjusting the iris diaphragm Lowering the stage Turning off the substage condenser light source Changing the objective lens2. Match each part to its function. Stage Stage adjustment knobs Light intensity knob Interpupillary adjustment Coarse focus knob Fine focus knob Iris diaphragm Condenser Condenser adjustment lever Specimen holder Eyepiece length adjustment (find the white ring) Electrical cord Closes down the amount of light, and sharpens image. Lifts or lowers the condenser; the closer to the specimen the better. Should be set so that both eyepieces are the same length. Moves a slide side-to-side and front-to-back. Changes lamp brightness. Should only be detached by holding the plug. Aligns light waves before they pass through specimen. Provides flat surface for specimens. Allows both eyes to see an image simultaneously. Holds a slide in place on the stage. Moves the stage up and down in tiny amounts. Moves the stage up and down rapidly.True or False 1. When focused on a specimen, the working distance when using the 4X lens is less than if you were using the 10X lens. 2. When focusing on the specimen using the 10X lens start with the fine adjustment knob. 3. Everything you see in the microscopic view is upside down and backward.
- 3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmKey Term Matching 1. stage A. the eyepiece 2. stage adjustment knob B. concentrates the light into specimen 3. fine adjustment knob C. opens and closes to regulate the light 4. course adjustment knob D. moves stage forward, back, left and right 5. nosepiece E. platform for the slide 6. light intensity control F. rheostat 7. condenser G. rotates to change objective lenses _8. slide holder H. used for precision focusing 9. ocular lens I. used to move the stage up or down 10. iris diaphragm J. hold the slide in place on the stage
- 1. Calculate the total magnification for the following: A. Scanning power objective = 5x; eyepiece =4x B. High power objective = 40x; eyepiece= 5x c. Low power objective = 10x; eyepiece= 10xCompute the total magnification. [Use 10x magnification for eyepiece]1. Scanning2. LPO3. HPO4. OIOblackboardcdn.com/5bfc08ba3fldc/14683296?X-Blackboard-Expiration=16245468000008X-Blackboard- 19 / 47 100% 1.4. Functions of the light mlcroscope parts Complete the following table by writing the function(s) of each of the parts indicated. Structure Function Diaphragm / iris Stage opehing Lamp Objective lenses Eye piece Coarse and find adjustment knobs Stage Stage rack prt sc delete home backspace lock enter pause t shift