DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases DNases
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- Which of the following enzymes has 5' to 3' exonuclease activity? DNA polymerase lII O Telomerase DNA ligase O Primase DNA polymerase lDNA fragments can be separated by: O gel electrophoresis O autoradiography O x-ray crystallography O centrifugationFormation ofa recombinant DNA molecule. GAATTC GAATTC CITAAG CITAAG double-stranded DNA DAATTO DAATTO CTTAA OG CTAA AATT GI AATT CTTAA GAARIC CTTAAG The enzyme that cataly zes the joining of fragments to form recombin ant DNA is: DNA polymerase DNA ligase helicase re striction endonuclease
- DNA pol I O is a single subunit enzyme has a 5' to 3' exonuclease activityWhich of the following enzymes do not require a DNA template for nucleotide synthesis? * O DNA polymerase I RNA polymerase O Primase O poly-A polymerase O DNA polymerase IIConsidening the structure of DNA, what kind of bonds hold one nucleotide to another within one side of the DNA molecule? O hydrogen O phosophodiester O lonic peptide
- Which of the following enzymes can recognize and remove a damaged DNA base to leave an apurinic or apyrimidinic site? DNA ligase RecA AP endonuclease DNA polymerase DNA glycosylaseThe discontinuous aspect of replication of DNA in vivo is caused by trinucleotide repeats lack of primase formation DNA polymerase slippage DNA supercoiling the 5' to 3' polarity restriction in DNA synthesis OOO OOComplete the complementary stand of the DNA shown Complementary strand стАG GTACT CAC G
- Which of the following is not necessary for replication to proceed? O MRNA O RNA primer O DNA polymerase O primase O DNA ligaseWhich of the following enzymes can recognize and remove a damaged DNA base to leave an apurinic or apyrimidinic site? DNA ligase DNA glycosylase AP endonuclease RecA DNA polymeraseThe enzyme is used to remove tension from the DNA double helix during replication called? Restriction endonuclease Gyrase DNA polymerase Helicase Ligase