A variety of organic chelating ligands have been synthesized totightly coordinate radioactive metal cations to identify and treat malig-nancies by coupling the metal complex to a polypeptide linker attachedto a monoclonal antibody that binds specifically to a cell surface re-ceptor. One such metal complex is illustrated in the diagram on theright. The radioactive Cu-64 cation is tightly coordinated by the che-lating ligand that is, in turn, conjugated to a peptide linker attached toa monoclonal antibody. (The antibody is not shown in this diagram.)When coupled to a specifically designed monoclonal antibody, thecomplex binds specifically to somatostatin receptors that are ex-pressed on the surface of neuroendocrine tumors. Subsequently theentire complex with the receptor is internalized, i.e., passed into thecytoplasm, where the radioactive metal cation kills the malignant cell.1.CH3HN NHNHHN NHNHHNНоНОNHHNS-SHNНоNHNHHN-NH HN-H2NOHNH) Write the three letter abbreviations and sequence numbers of the amino acid residues joined(d) (through the disulfide bridge. If this bridge were found for the two joined amino acids free in solution,what is the special name given to this molecule? Are the sulfur atoms in the disulfide bridge in anoxidized or a reduced state?

Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Answered: (d) ( through the disulfide bridge. If…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Certain naphthalene derivatives, such as the dansyl group, exhibit a weak yellow fluorescence when they are in a highly polar environment (such as water) and an intense blue fluorescence when they are in a markedly nonpolar environment (such as hexane). The binding of ´ -dansyl-lysine to specific antibody is accompanied by a marked increase in its fluorescence intensity and a shift in color from yellow to blue. What does this finding reveal about the hapten–antibody complex?
Cadherins are known to mediate homophilic interactions between cells. What is a homophilic interaction, and how can it be demonstrated experimentally for E-cadherins?
The protein fragments ABS1 and ABS2 of tropomodulin were produced as fusion proteins with chitin binding domain and purified by a chitin column. Explain the principles of this type of affinity chromatography and support it with a self‐drawn figure. This is with regards to Tropomodulin/ F-Actin complex.
Azathioprine is an immunosuppressant drug used in kidney transplants. In vivo, azathioprine is metabolized to the hypoxanthine analog 6- mercaptopurine, which is then converted into 6-mercaptopurine ribose monophosphate, the active form of the drug. 6- Mercaptopurine ribose monophosphate also inhibits de novo purine synthesis, reducing uric acid levels in the blood and urine. However, when administered to Lesch – Nyhan patients, there was no effect on uric acid levels. Explain why this is the case.
1. A variety of organic chelating ligands have been synthesized to tightly coordinate radioactive metal cations to identify and treat malig- nancies by coupling the metal complex to a polypeptide linker attached to a monoclonal antibody that binds specifically to a cell surface re- ceptor. One such metal complex is illustrated in the diagram on the right. The radioactive Cu-64 cation is tightly coordinated by the che- lating ligand that is, in turn, conjugated to a peptide linker attached to a monoclonal antibody. (The antibody is not shown in this diagram.) When coupled to a specifically designed monoclonal antibody, the complex binds specifically to somatostatin receptors that are ex- pressed on the surface of neuroendocrine tumors. Subsequently the entire complex with the receptor is internalized, i.e., passed into the cytoplasm, where the radioactive metal cation kills the malignant cell. CH3 HN NHNH HN NHNH HN НО НО NH HN HN HO S-S NH (a) dues in the polypeptide. Place the label…
Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB? What is the equation for the double-reciprocal plot at 20 nMnM NAMB?
Which letter represents the target site of furosemide (Lasix)?
Monoclonal antibodies can be conjugated to an insoluble support by chemical methods. Explain how these antibody-bound beads can be exploited for protein purification.
The ACE2 receptor protein in humans has been getting a lot of press these days, as it apparently serves as a "receptor" for the SARS-CoV-2 coronavirus in addition to its normal functions. This protein has an isoelectric pH of 5.36. Suppose you want to purify this protein to study its properties, with the goal of developing a drug that would block it from binding the virus. Once you have purified the cell surface proteins from your cultured human lung cells away from all the other types of molecules in the cell, and all the cytoplasmic proteins, you might decide to use ion exchange chromatography to separate the ACE2 proteins from other proteins in your sample. If you plan to load your protein mixture onto your ion exchange column in a buffer at pH 7.4 (approximately physiological pH) and you want the ACE2 proteins to bind to the column, should you use a resin with a positive charge or a negative charge? Briefly explain why you chose the resin you selected.
b) Consider the following experimental results: Total hydrolysis of a nonapeptide gave: Ala,Arg, Glu,Leu,Lys,Met,Phe,Tyr,Val One fragment from CNBR treatment was: Tyr-Leu-Lys One fragment from Chymotrypsin treatment was Arg-Glu-Met-Tyr One fragment from Trypsin treatment was Val-Ala-Phe-Arg i. Deduce, with suitable explanations, the amino acid sequence of the nonapeptide.
What are the basic properties of e-cadherins? Namely, what is the basic structure of specifically e-cadherins, what affects their function (e.g. Ca2+), their ligand activity, and their role in cell sorting? Be specific/distinct with every point here.
Crystal structures exist for three neurokinin-1 (NK1) ligand complexes with the following pdb codes (6hll, 6hlo,6hlp). State which is the highest quality crystal structure indicating the criteria you used to evaluate this.

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