Calculate the number of bacteria (CFU/ml) in each serum and saline sample. What was the purpose of the saline? Which bacteria produced more CFU/ml in serum? Why? Provide a possible explanation for the ability of Pseudomonas to colonize mucous membranes. Reference(s): Include at least 1 valid reference for your research in correct format (either APA or MLA) (2pts)
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- Calculate the number of bacteria (CFU/ml) in each serum and saline sample.
- What was the purpose of the saline?
- Which bacteria produced more CFU/ml in serum? Why?
- Provide a possible explanation for the ability of Pseudomonas to colonize mucous membranes.
- Reference(s): Include at least 1 valid reference for your research in correct format (either APA or MLA) (2pts)
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- A patient with AIDS is hospitalized with symptoms of high fever and rigidity of the neck. Routine laboratory tests on the CSF show a WBC count of 100/m L with a predominance of lymphocytes and monocytes, glucose of 55 mg/dL (plasma: 85 mg/dL), and a protein of 70 mg/dL. The Gram stain shows a questionable starburst pattern. Questions: a. What additional microscopic examination should beperformed?b. If the test is positive, what is the patient’s diagnosis?c. If the results of the test are questionable, what additionaltesting can be performedAs a medical microbiologist, if you are presented with a patient suspected to have a bacterial disease, describe the various steps you would follow, to establish a laboratory diagnosis. Please keep brief - 8 sentences/dot points max.You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flask
- Explain the following tests using Pseudomonas aeruginosa. Describe the purpose, reagents used and the results using Pseudomonas aeruginosa.Answer the following questions: 1. What was the first antibiotic and what was its importance? 2. What does resistance mean? 3. Who is affected by resistance? 4. What if the resistance problem is not solved? 5. Describe the structure of the bacterium (its parts) 6. Can bacteria change? explain 7. Why do Bacteria communicate, what is the purpose? 8. Explain how a bacterium achieves its resistance. 9. What is the use given to antibiotics in production animals? 10. Is this use in animals good practice? 11. Once resistance occurs, what has the scientific community had to do? 12. Do antibiotics only affect negative bacteria? explain. 13. What are the most feared diseases due to antibiotic resistance? 14. Should antibiotics be used against viruses? explain. 15. How can we avoid antibiotic resistance?What is the microbiology laboratory test that Identifies Pseudomonas putida and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.
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