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- Make use of the table below in answering the questions asked: Amino acid pK₁ pK₂ pK, Isoleucine 2.32 9.76 Leucine 2.32 9.74 Lysine 2.16 9.06 10.54 Tyrosine 2.20 9.21 10.46 1. Kindly draw manually the structure of tripeptic ILY at pH 7.00 2. Kindly draw manually the most protonated structure of tripeptide ILYThe peptide aspartyl-glutamyl-leucyl-threonyl-alanine, shown in the sketch drawing window, has several ionizable groups. Adjust the charges to show the molecule as it would exist at pH 5.00. Use the pk values in the table below. pK, Values for Ionizable Groups of Amino Acids. Amino Acid pA, of a-COOH pA, of e-NH, pA, of Side Chain Isoelectric Point (pI) Alanine 235 9.87 6.11 Arginine 2.01 9.04 12.48 10.76 Asparagine 2.02 8.80 5.41 Aspartic acid 210 9.82 3.86 298 Cysteine 2.05 10.25 8.00 5.02 Glutzmic acid 2.10 9.47 4.07 3.08 Glutamine 217 9.13 5.65 Glycine 235 9.78 6.06 Histidine 7.11 9.18 6.10 7.64 Isoleucine 232 9.76 6.04 Leucine 233 9.74 6.04 Lysine 218 8.95 10.53 9.74 Methionine 2 28 9.21 5.74 Phenylalanine 2 58 9.24 5.91 Proline 2.00 10.60 6.30 Serine 221 9.15 5.68 Threonine 2.09 9.10 5.60 Trуptophan 238 9.39 5.88 Tyrosine 2 20 9.11 10.07 5.63 Valine 2 29 9.72 6.00 • Use the wedge hash bond tools to indicate stereochemistry where it exists. • If a group is 2chiral, do not use…Place each charge form of alanine under the pH condition where it would be the predominant form. The pK₁ values for the carboxyl group and amino group of alanine are approximately 2.3 and 9.7, respectively. pH 11 H | H₂C-C-COO NH₂ Does not occur in significant amounts at any pH
- The pK, of the α-carboxyl group of serine is 2.21, and the pK, of its α-amino group is 9.15. Calculate the average net charge on serine if it is in a solution that has a pH of 9.00. average net charge on serine:Five amino acids were subjected to reverse phase chromatography, and the following Rf values were computed: A - 0.23 B - 0.08 C - 0.54 D - 0.74 E - 0.96 Which is the most polar amino acid? Group of answer choices E D B C AA mixture of Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74), Phenylalanine (pl 5.48) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2. v First 1. Aspartic Acid v Second 2. Histidine v Third 3. Lysine 4. Phenylalanine v Fourth 5. Threonine v Fifth 6. No separation
- a) Circle or highlight with yellow coloring each essential amino acid residue that appears in the hirudin sequence. b) The prototropic groups and their stoichiometry in hirudin are listed below. Calculate the charge on hirudin at pH values 3 and 4 and then estimate its isoionic point.A 100 mL of 0.1 M amino acid at pH 1.0, whose pka for the carboxyl group is less than the pKa of the R-group, was titrated with NaOH solution. The pH was monitored, and the results were plotted on a graph, as shown below. The key points in the titration are designated A to G. 12 10 8 pH 6 4 2 0 A B 0.5 с D E F 1.5 2 2.5 Equivalents of OH 1. What is the possible identity of the amino acid? [Select] 2. What is the isoelectric point of the amino acid? [Select] 3. What is the pka corresponding to the deprotonation of the alpha-amino group? [Select] 4. Region/ point where the amino acid is predominantly present as a (-2)-charged species. [Select] 5. The effective buffering range for the amino acid in the basic region. [Select] G 3Assume a pH of 7.0 in aqueous solution. Please draw a tripeptide containing (in this order) a residue with a residue with an non-polar sidechain containing more than five carbons, a positively (+1) charged sidechain, and a residue with a polar (but not charged) sidechain with more than two carbons. Please give the names of the amino acid residues that you drew.
- A peptide was treated with Sanger's reagent followed by hydrolysis with 6M HCl for 24 hours at 105 oC. Chromatography of the mixture identified Val and Lys as the DNB labeled amino acids. Assuming a single peptide chain, explain how this could be and which is likely the actual N-terminal amino acid.You have discovered a new amino acid that you name in honor of your favorite course. Twofourteeninine has an alpha-amino and an alpha-carboxylic group, and an ionizable side chain that carries an amino group. A titration curve of twofourteeninine is shown below. Net charge a P I 1 2 I 3 4 5 6 pH T 9 10 11 12 13 What are the approximate pKa values of twofourteeninine (to the nearest half-pH unit)? Check all that apply.A mixture of Alanine (pl 6.02), Glutamic Acid (pl 3.22), Glycine (pl 5.79), Lysine (pl 9.74) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2 ? v First 1. Alanine v Second 2. Glutamic Acid v Third 3. Glycine v Fourth 4. Lysine v Fifth 5. Threonine 6. No separation