A 1 mL sample of glycogen was calculated to contain 21 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCl. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 340 µg/mL (microgram/milliliter).i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1 mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample.ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample.iii) Calculate the purity of the glycogen used in the sample as% Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100iv) state your answer in a complete sentence.Show your working out such that the marker can easily understand it. Include all units. Assume the molecular mass of glucose is 180 g/mol. At each step round off your calculations to 1 decimal point.

This question is from basic mole concept .

Answered: A 1 mL sample of glycogen was…

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter10: Fuels, Organic Chemicals, And Polymers

Section: Chapter Questions

Problem 119QRT

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A 1 mL sample of glycogen was calculated to contain 21 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCl. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H₂O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 340 µg/mL (microgram/milliliter).

i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1 mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample.
ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample.

iii) Calculate the purity of the glycogen used in the sample as

% Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100

iv) state your answer in a complete sentence.

Show your working out such that the marker can easily understand it. Include all units. Assume the molecular mass of glucose is 180 g/mol. At each step round off your calculations to 1 decimal point.

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