5. When used for imaging, the wavelength of the radiation needs to be smaller than the thing being imaged. To a get a pretty good idea of what kind of radiation is needed to image something assume that the wavelength must be the size of the object or smaller. With this in mind, would visible light work well to image protozoa with a length of 10 micrometers? What about bacteria (about 1 micrometer)? How about a virus (about 0.1 micrometer)?

5. When used for imaging, the wavelength of the radiation needs to be smaller than the thing being imaged. To a get a pretty good idea of what kind of radiation is needed to image something assume that the wavelength must be the size of the object or smaller. With this in mind, would visible light work well to image protozoa with a length of 10 micrometers? What about bacteria (about 1 micrometer)? How about a virus (about 0.1 micrometer)?

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter4: Cell Structure

Section: Chapter Questions

Problem 4RQ: When viewing a specimen through a light microscope, scientists use to distinguish the individual...

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1. Why is it helpful that the transmission compound light microscopes we studied in lab are both parfocal and parcentered? O Why would it be harder to find a specimen on a slide starting with the 40x objective lens compared to the 4x objective lens? Support your answer with facts about the field of view and the comparison of both objective lenses. If I wanted to observe something, let's a virus that was 50 nm in size which microscope would you recommend me using? Support your answer with facts about the microscope's utility.
1. Typical microorganisms are on the scale of 1 micrometers. The typical human eye can see things as small as 0.1 millimeter unaided. With a standard microscope, you can see things on a scale of 100 nanometers. (a) Can you see microorganisms with your eye? With a microscope? Justify your an- swer by writing everything in scientific notation. (b) How many times magnified does a standard microscope make something look, as compared to a bare eye?
7. What determines the resolution limit of a microscope? Is the purpose of the fluorescence microscope to increase the magnification beyond that of a typical light microscope? Why or why not? d in lah.
1. Is a microscope an absolute necessity for studying microorganisms? Why or why not? 2. What do you think are the limitations of a brightfield microscope? Why is a brightfield microscope the most common type of microscope in routine clinical laboratories? 3. A slide in which a stained specimen is placed on the microscope stage. After careful and correct manipulation of the adjustment knobs, the specimen is still not in focus. What do you think is the reason? What corrective actions can be done? (provide computation) 1. What is the formula to determine the actual size of an organism? 2. The actual size of an Escherichia coli is 1.5um. What would be its image size if the magnification used is 1000x? 3. What magnification is used if an Bacillus sp. have a microscopic size of 3.5um and an image size of 15mm? NOTE: please try to answer all of the question, i promised to give you a good ratingg
1. Why is nanotechnology likened to creating a statue out of apile of dust?
1. What magnification does the microscope need to be able to see cells? 2. What magnification does my microscope need to see bacteria? 3. What are the different magnifications on a microscope? How do I calculate total magnification?
1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
4. If the diameter of the field of view (FOV) for your microscope is 5.5 mm at 40x total magnification, what is the diameter of the FOV at 1000X? Show work and don't forget units! 5. Use the counting method to estimate the length of an elodea cell. You will need to use your calculation for the FOV at 400x total magnification. Show work and don't forget units! Elodea Leaf at 400x total mag 6. Use the percent method to estimate the size of a human cheek epithelial cell. You will need to use your calculation for the FOV at 1000x total magnification. Show work and don't forget units! Cell Membrane Nucle us
4. Consider the following microscope: power = 10X power objective = high power objective 50X ocular low What is the highest magnification you could get using this microscope? If 10 cells can fit end to end in the low power field of view, how many of those cells would you see under high power?
2. The objective lens allows you to zoom in to the microscope slides. The microscopes that we use in the classrooms normally have three lenses with the following magnifications: 10x, 40x, 100x. Write the correct magnification under the images.
1. How are electron microscopes different from light microscopes and how is their ability to observe a specimen differently? 2. Explain why both magnification and resolution are important in order to see a microscopic organism? 3. When using a light microscope, focus the specimen with the scanning objective lens first? explain your answer?
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