2. Match the following molecules with the-corresponding test reagents that are used to identify them. Also, indicate the positive test color and what reagent component reacts with what specific chemical group that is responsible for a + test result Protein D Starch C Amino Acids B Sugar G Lipids A DNA F RNA E A. Sudan IV B. Ninhydrin C. Iodine D. Biuret E. Orcinol F. Diphenylamine G. Benedict
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- 49. I- Additive comes in two forms, liquid and spray-dried coated inside the test tube. Il- K2 EDTA is an example of liquid form O A. If First statement is CORRECT and second is WRONG O B. If First statement is WRONG and second is CORRECT C. If BOTH statements are CORRECT O D. If BOTH statements are WRONG 50. I- The needle in the butterfly method is shorter than the hypodermic needle in syringe method. Il-The length of the needle is approximately 1.27 cm to 2 cm O A. If First statement is CORRECT and second is WRONG O B. If First statement is WRONG and second is CORRECT O C. If BOTH statements are CORRECT ODIF BOTH statements are WRONG1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutesDraw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR 1. Bring the test reagents and samples to room temperature (Note 1). 2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing. 3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop of positive control serum and 1 drop of negative control into two additional circles. 4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested. 5. Mix the contents of each circle with a disposable stirrer while spreading over the entire area enclosed by the ring. Use separate stirrers for each mixture. 6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note 2). 7. Observe immediately under a suitable light source for any degree of agglutination.
- 1. a 1 mL stock sample is added to 9 mL Of diluent to make solution A, and then 2 mL of solution A is added to 18 mL of diluent to make solution B. what is the final dilution with respect to the stock? 2. in most probably number (MPN) testing what media is used for confirming test and what does a positive result looks like? 3. In Most probably number (MPN) testing what can be inferred from a positive result on the presumtive test?Draw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.Chemical tests See bottom of page 8 for instructions on how to complete this table. C. Biuret test Solution turins olive greeni Yeu ight Pur ple A. Iodine test B. Benedict's test Description of Solution turne Slohtion turns positive result Tube No. and (copy from Contents dark Purple or brovniornd asbiutrldeins Pp. 7-8) decp blue-blakk or tomoto of Short +/- Appearance blue (from p. 9) らish | +/- | Appearance blae Appearance +/- 1 - Orense 2 - Glucose Starch orunde Orange 3 - ders Brtelt blue blue blve bue 4 - alanine oransC- blue - 5. blue iout bue t orange Why was it important to have tube number 1 in each test? (Don't just say "because it's the control", and don't copy from the lab instructions-write out your own understanding of why and how that tube was used.)
- Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive test1. Compare and contrast DAT from IAT based on procedure and clinical significance. 2. Explain the importance of the three phases of Indirect Coomb's test. 3. Differentiate the three potentiators used in Coomb's Test (PEG, LISS, 22% BSA)what is ASTO or ASO test? discuss the principle it's important
- Draw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 ml of 1X diluent or saline in a test tube. Pipet 50 µul of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.1. Conclude regarding the chemical composition of the formation of film in fresh milk in biuret test and acrolein test.A. Experimental Results Sample No. 1 2 3 4 5 1 Sample No. Sample Identity 2 3 + Table 1. Results of the Qualitative Tests of Unknown Lipid Samples Copper acetate Test Greenish-blue color Colorless Colorless Greenish-blue color Colorless 5 Acrolein Test Hubl's Test Ammonium Molybdate Test No precipitates No precipitates No precipitates No precipitates Yellow precipitates No distinct odor Pungent odor No distinct odor No distinct odor Pungent odor Brown solution Light pink color Brown solution Light pink color Light pink color Table 2. Sample Identity of Unknown Lipid Samples Rationale Liebermann- Burchard Test No color change No color change Bluish-green color No color change No color change