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- Desired Desired Initial Volume of Stock or Volume of Concentration Volume Concentration Previous Dilution Distilled Water (C2) (V2) (C1) (V1), µL (V2- V1), µL 100 mg/dL 1.2 mL 200 mg/dL 75 mg/dL 1.2 mL 100 mg/dL* 50 mg/dL 1.2 mL 25 mg/dL 1.2 mLSuspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.Calculate the amount of each solution needed to make 750 mL if a dextrose 25% solution given only a Dextrose 60% solution and a Dextrose 5% solution This is the full question
- Explain how you will prepare 100 cfu/mL bacterial suspension. Present in diagram if necessary and show all calculations involved.Calculations: Explain how you will prepare 100 cfu/mL bacterial suspension. Present in diagram if necessary and show all calculations involved.50 mL of 5mM NaCl solution from 1 M NaCl solution
- Administer Reglan 10mg in 100mL IVPB to run over 30mins The drip factor is mL .Calculate the concentration of the overnight stock culture in CFU/mLI have another one, 1 ml of sewage water is added to 9 ml of water. 0.1 ml of this is added to 9.9 ml of water. 1 ml of this is then added to 9 ml of water. 1 ml is plated and 243 colonies form. What is the CFU/ml of the sewage water? Thanks again.
- Why are we using this materials and centrifuging at 15000 rpm? Lipid peroxidation experiment:Materials;• 10% TCA solution• 0.67% TBA solution• Tissue sample Procedures:The tissue sample is buffered at the ratio of 1 x 4 and crushed with the help of a blender. It is centrifuged at 15 000 rpm. Then, 2.5 mL of 10% TCA is added onto 0.5 mL of tissue sample and the tubes are mixed in vortex. It is kept in boiling water for 15 minutes and immediately cooled. After centrifugation at 5000 rpm for 15 minutes, 2 mL of each supernatant is transferred to another tube. 1 mL of 0.67% TBA is added on it and mixed in vortex. After the samples are kept in boiling water for 15 minutes and cooled immediately, their absorbance at 532 nm is recorded. The amount of MDA is calculated by utilizing the highest absorbance specific absorbance values (E = 1.56 x 105cm-1M-1 ) of the MDA-TBA complex formed at 532 nm.What is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)