Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1 ml 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample= number of bacteria/ml (For example, if 32 colonies are on a plate of ¹/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright ©2004 Pearson Education, Inc., publishing as Benjamin Cummings
Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1 ml 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample= number of bacteria/ml (For example, if 32 colonies are on a plate of ¹/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright ©2004 Pearson Education, Inc., publishing as Benjamin Cummings
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 23RQ
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