Question 15 When you successfully inserted a gene fragment into the Hindill site and transform bacteria with the plasmid. How can you tell which transformants have the insert? The plasmid PSU922 is a circular DNA containing 25000 base pairs. The B-gal gene codes for the enzyme ß-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpⓇ gene confers ampicillin resistance. EcoRI Bam HI BamHI HindIII PSU922 BamHI Bam HI EcoRI ori C A The bacteria will not be able to grow in the presence of ampicillin, and they will be blue. (B) The bacteria will not be able to grow in the presence of ampicillin, and they will be white. C The bacteria will be able to grow in the presence of ampicillin, and they will be white. The bacteria will be able to grow in the presence of ampicillin, and they will be blue. AmpR B-gai

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A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.