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- QUESTION 8 Consider the pathway for the synthesis of the amino acid arginine in Neurospora: ARG-E → citrulline ARG-F ARG-H ornithine argininosuccinate arginine Mutant strains of Neurospora may carry one or more mutations. Neurospora mutant strain b is grown on minimal media plus supplements as shown. Growth is shown by (+) and no growth is shown by (o). Supplements ornithine mutant nothing citrulline arginino- succinate arginine strain What can you conclude from these data? O Strain a has only one mutation and it is in ARG-E. O Strain b has only one mutation and it is in ARG-H. O Strain a has a mutation in ARG-F and strain b has a mutation in ARG-E. O Strain b has mutations in ARG-E, ARG-F, and ARG-H. + +Question 1 options: The specificity pocket of the serine protease chymotrypsin, which interacts with Tyr and Phe-containing peptide sequences, contains a Ser residue. A research group is trying to modify chymotrypsin such that it has a low KM with Trp-containing peptides. Enter the name or abbreviation of an amino acid that the Ser could be mutated to that would likely have the desired effect. (Hint: look at the diagrams of the specificity pockets shown in the course slides, and consider how the Ser would need to change to account for the difference between Tyr/Phe and Trp.)Question 1 A 60-year-old woman was recently given a diagnosis of advanced non- small cell lung cancer. She is going to begin treatment with cisplatin 100 mg/m 2 plus vinorelbine 30 mg/m2. Discuss your pharmacological plan
- Question:- 1. Describe an assay that could measure the activity of adenylyl cyclase (AC).QUESTION 1: Several factors (e.g., time and voltage) affect migration of DNA fragments through the agarose gel. Briefly explain how each of these factors affects DNA migration.Question 4: Imagine that you isolated X-receptor proteins from mutant cells with the nonsense mutation in exon 3. You also isolate X-receptor proteins from wild-type (normal) cells. If you ran these protein molecules on a gel which are your predicted results? A В C Ladder Wild-type Mutant Ladder Wild-type Mutant Ladder Wild-type Mutant
- Question 14 Why are long reads the preferred method to sequence repetitive elements? Select the statement that is FALSE. ◇ If a repetitive region is longer than 300 basepairs (the length of a short read) it's impossible to determine the total length. O Long reads are more likely to span repetitive regions on either side, making it easier to map the repetitive regions. O It's hard to determine where short reads should be placed, as they could map to multiple repetitive regions. O Long reads are less error prone and thus better for sequencing overall. Question 15 Which of the following statements is NOT true about GWAS? When identifying alleles associated with a disease, we compare the frequency of an allele in a control group versus a cash groupQUESTION 16 Which of the following best describes the proper sequential order of the dogma of molecular genetics? O A. Deoxyribonucleic acid is converted to a pre-messenger ribonucleic acid that is modified to a messenger ribonucleic acid that is converted to a polypeptide chain, which is modified to a protein. B. Nucleic acid is converted to a pre-messenger ribonucleic acid that is modified to a messenger ribonucleic acid, which t is converted to a polypeptide chain that is modified to a protein. OC. Nucleotide is converted to a messenger ribonucleic acid that is modified to a polypeptide chain, which is modified to a protein. O D. Polynucleotide is converted to a pre-messenger ribonucleic acid that is modified to a messenger ribonucleic acid that is converted to a polypeptide chain, which is modified to a protein. O E. None of the answers (A, B, C, and D) is the correct answer choice for this question. QUESTION 17 Which of the following statements is precisely correct/true? O A.…Question 1: Your research project is concerned with the expression and purification of a protein called aldehyde dehydrogenase. Describe in detail all steps required from gene amplification to characterisation of the purified protein.
- An experiment was conducted with two groups of genetically identical female agouti mice (Avy/a) that were mated with normal (a/a) male mice . Both female groups ate lab chow with bisphenol A (BPA) (50mg/Kg). (BPA, used in the manufacture of polycarbonate plastics, has estrogenic effects that include decreased CpG methylation.) The lab chow of one group was supplemented with methyl donors (e.g., folate, choline, and vitamin B12). The effects of BPA were abolished in the Avya offspring of the mice fed with the methyl donor–supplemented chow (i.e., the coat color distribution observed was shifted toward brown). Explain.question 26 What is true for CpG Islands: stretches of a few hundred base pairs of DNA where cytosines are unmethylated are not associated with genes are not found around the promoters are associated with silenced genesQuestion 16 Concerning SDS-PAGE: |A The gel is a copolymer of agarose and bis- agarose. BA magnetic field drives the migration of the proteins within the gel. C Is usually performed in presence of a molecular weight marker composed of a mixture of polynucleotides of known molecular weight. D The gel behaves like a molecular sieve. E In this type of electrophoresis the proteins migrate from the positive (+) to the neg- ative (-) electrode.