Design a transcriptional circuit that regulates the expression of β-galactosidase in a eukaryotic cell, such that the enzyme is produced in the presence of lactose and repressed in the absence of lactose. Use at least one of the following elements: histone acetyltransferase, histone deacetylase, chromatin remodeling complex

Design a transcriptional circuit that regulates the expression of β-galactosidase in a eukaryotic cell, such that the enzyme is produced in the presence of lactose and repressed in the absence of lactose. Use at least one of the following elements: histone acetyltransferase, histone deacetylase, chromatin remodeling complex

Name: Design a transcriptional circuit that regulates the expression of β-ga
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: Design a transcriptional circuit that regulates the expression of β-galactosidase in a eukaryoticcell, such that the enzyme is produced in the presence of lactose and repressed in the absenceof lactose. Use at least one of the following elements: his

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter16: Gene Expression

Section: Chapter Questions

Problem 18RQ: IS. Alternative splicing has been estimated to occur in more than 95% of multi-exon genes. Which of...

See similar textbooks

Similar questions

Transcription factors function in the nucleus. However, like (almost) all eukaryotic proteins,they are translated in the cytosol. Can you draw a visual to explain how transcription factor proteinsenter the nucleus from the cytoplasm? Can you also include a representation of relevant proteins and proteindomains to explain how these proteins reach their destination. Thank you
ATM is a kinase that phosphorylates histone H2AX in response to double-stranded DNA breaks. Which of the following scenarios would most quickly regulate ATM activity in the cell? a) Adding silencing methyl groups to cytosines in the Atm gene b) Modifying the histone code for the Atm gene c) Increasing expression of a miRNA specific for the Atm mRNA d) Activating an E3 ubiquitin ligase specific for the ATM protein
Histone methylation can have many different effects on gene expression. In some cases, histone methylation is associated with activation of transcription, whereas in other cases it can trigger the formation of heterochromatin and a decrease in transcription. If histone methylation has been detected in the region of gene YFG in yeast, describe an experiment that could distinguish whether the methylation is important to activate or repress transcription of gene YFG.
What effect would inhibitors of histone deacetylases have upon transcription? Group of answer choices They would increase transcription by making the chromatin more compact They would increase transcription by making the chromatin less compact They would decrease transcription by making the chromatin more compact They would decrease transcription by making the chromatin less compact For this question, we will consider a eukaryotic mRNA that has four exons (E1, E2, E3, E4) and three introns (I1, I2, I3). What could occur if a protein were to bind over the 3' splice site of intron 2 (I2)? Group of answer choices The processed mRNA would consist of: E1+E2+E3+E4 The processed mRNA would consist only of: E1+E3 The processed mRNA would consist only of: E3+E4 The processed mRNA would consist of: E1+E2+E4
Identify which eukaryotic level of gene regulation is most applicable in the given examples. (Pre-transcriptional control, Transcriptional control, Translational control, Post-translational control) The glycolytic enzyme pyruvate kinase is activated by dephosphorylation and inactivated by phosphorylation. In the fruit fly, genes for rRNA can be replicated more or less often compared to the rest of the chromatin depending on the needs of the cell. In the human beta-globin, two introns are spliced out in order to produce the mature mRNA. DNA methylation can change the degree of condensation of the chromatin.
You are teaching a class on the regulation of eukaryotic gene expression. In order to demonstrate this complex process, you decide to draw for the class a typical eukaryotic gene/transcription unit with its major regions, such as the promoter regions, where the RNA polymerase II and transcription factors would bind From the list given - choose all components that you think are part of a typical eukaryotic gene From the list given - choose all the regulatory sequences that you think would control the expression of this eukaryotic gene From the list given - choose all of the regulatory proteins that would bind the eukaryotic gene to control its expression
Searching the yeast Saccharomyces cerevisiae genome, researchers found approximately 4,000 DNA sites with a sequence which could potentially bind the yeast transcription factor GAL4. GAL4 activates the transcription of galactose genes. Yet there are only 10 GAL4-binding sites which control the genes necessary for galactose metabolism. The GAL4 binding sequence is CGGAT#AGAAGC*GCCG, where # is T, C or G, and * is C or T. In one chromatin immunoprecipitation experiment (ChIP), yeast growing on galactose were lysed, and subjected to cross-linking reagents which cross-linked transcription factors and activators to DNA. Next the DNA was sheared into small fragments, and antibodies to GAL4 were added. These antibodies coprecipitated the GAL4 and the DNA it was cross-linked to. The cross-linking was then chemically reversed, and the DNA was isolated, cloned into a library of plasmids and sequenced. Results showed that only 10 different DNA sequences had GAL4 bound. Since the…
Gene X codes for a protein in eukaryotes. A mutated eukaryotic cell contains an altered base-pair in an intron of gene X. Which would be the most likely effect of this mutation on the biomolecules in the cell? The amount of pre-mRNA transcribed from gene X would be less than normal. The amount of functional protein corresponding to gene X would be less than normal. The ability of snRNAs to form a spliceosome would be diminished. The breakdown of mature mRNA corresponding to gene X would be faster
Help me please
Typically, increased acetylation and decreased methylation of histone N-terminal tails in nucleosomes leads to removal of the protein components of the chromatin from the DNA relaxed packaging of the chromatin and increased transcription increased amounts of euchromatin relative to heterochromatin activation of topoisomerase tighter packaging of the chromatin and reduced transcription
Like Hurler syndrome, Fabry disease involves an abnormal accumulationof substances within lysosomes. However, the lysosomes of individuals with Fabry disease show an abnormal accumulation of lipids. The defective enzyme is α-galactosidase A, which is a lysosomal enzyme that functions in lipid metabolism. The defect causes cell damage, especially to the kidneys, heart, and eyes. The gene that encodes α-galactosidase A is found on the X chromosome. Let’s suppose a phenotypically unaffected couple producestwo sons with Fabry disease and one phenotypically unaffecteddaughter. What is the probability that the daughter will have anaffected son?
Which of the following are examples of post- translational modification (PTM)? (select two answers) Genomic DNA is methylated by a DNA methyltransferase, altering the binding of transcription factors. Glycogen phosphorylase becomes active when phosphorylated by glycogen phosphorylase kinase. Glucose is phosphorylated by hexokinase as the first step in glycolysis. Ras is active when bound to GTP, and inactive when bound to GDP. Caspase 3 is activated when cleaved by capsase 9.