describe the structural features of protein transaminase/aminotransferases (primary, secondary, tertiary, or quaternary)?
Q: Which of the following lipids is NOT found in biological membranes? glycolipids…
A: Glycolipids are substances expressed on the surface of cellular membrane. Glycolipids are lipids…
Q: Thiophene ring contains ------- as heteroatom.
A: Thiophene is a compound with ring structure, in which there will be atoms of two or more different…
Q: Millon's test is used to detect the amino acid, tyrosine. However, to solely use the qualitative…
A: The amino acid tyrosine has a phenolic group in its side chain.
Q: How many chromosomes are there in a body cell of a person with trisomy 21 condition? Select one: О…
A: Trisomy 21 is a genetic chromosomal disorder . Genetic chromosomal disorders are the diesese occurs…
Q: In the RBCs of the patient described in the picture, which of the following would be expected?…
A: Glycolysis occurs in both aerobic as well as anaerobic respiration. Glycolysis is the only…
Q: Pyruvate can be processed under anaerobic conditions to ethanol (in yeast) or to lactate (in…
A: Introduction: Pyruvate is the product of glycolysis which is an important junction point in…
Q: 7. What is represented in the following diagram? Pyruvate Lactate Acetyl-coA dehydrogenase…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: Which among the following The colored solution formed as a positive result for the Biuret test is…
A: A polypeptide chain has amino acids linked together by a peptide backbone.
Q: The peptide below contains how many amino acid residues?
A: The name 'amino acid' is derived from the amine and carboxylic acid groups, and so these two parts…
Q: Reset Help The protein appears to have regions that are closely associated with the region between…
A: TAL effectors are transcription factors secreted by bacterial pathogens. They are virulence factors…
Q: J A positive result for Fohl's test is the formation of a black precipitate in the form of lead. O A…
A: Fohl's Tests - This test is performed to detect the presence of amino acid which containing sulfur.…
Q: What are massive, insoluble, high molecular weight compounds that have brown color? Not…
A: The brown pigment observed in different foods such as cookies, breads etc is the result of a…
Q: Restriction digestion of DNA fragments is not sequence specific. True False
A: Within a few years after discovering EcoB, EcoK, and HindII, scientists were already experimenting…
Q: Does oxidase, catalyzes the oxidation of molecular oxygen? True or False
A: Enzymes are biological catalysts that are proteins. Chemical reactions are sped up with catalysts.…
Q: Сно сно CH,OH Fo но H- HO- H- Phosphorogluco- isomerase Phosphofructo- kinase Нехоkinase но H- но…
A: The given pathway represents the glycolytic pathway, through which the glucose molecules are…
Q: Five sweetener samples (labelled A to E) were tested by 97 individuals for the intensity of their…
A: Carbohydrates or carbs are maconutrient consisting of Carbon, hydrogen and oxygen atoms. In nature…
Q: What are two functions of the hepatic portal system?
A: In the animal's circulatory system, there is presence of a portal venous system. This occurs when…
Q: A glycosidic bond between two monosaccharides can also be classified as a(n) ________
A: Monosaccharides are the basic sugar unit of carbohydrates compounds. Monosaccharides which cannot be…
Q: do aklaloids minic neutrotransmitters in our bidy? amines contain: a) nitrogen atom b) an acid c)…
A: 1) Ans- a)Nitrogen group. Explanation- Amine is Nitrogen containing compound. There are primary…
Q: A buffer can protect against changes in pH when hydrogen ion or hydroxide ion is added to a reaction…
A: Buffer solution is two types, Acidic buffer - this is composed of a weak acid and it's conjugate…
Q: What is the name of the predominant amadori molecule product in the binding of hemoglobin and…
A: Excess glucose can react nonenzymatically with the amino groups of intracellular and extracellular…
Q: Chain Shortening of monosaccharides can be done in the laboratory thru Kiliani-Fischer reaction.…
A: Carbohydrate chain shorting and lengthening is the process of increasing or decreasing the length of…
Q: In olfactory neurons, it is estimated that activation of the olfactory receptors results in an…
A: The olfactory epithelium of the nasal roof contains olfactory receptors. Each olfactory receptor is…
Q: D. Lactate
A: Glycolysis is the phenomenon in which sequence of reactions converted glucose to pyruvate and…
Q: Sample pH vs Initial Velocity (AA450/60 seconds) a bo16 0.0014 al0012 0.0008 0.0006 0.0004 0.0002 pH…
A: Enzymes are biological catalysts that are capable of catalysing a reaction by binding to Substrate.…
Q: • What happens to the functionality of a denatured enzyme? • How can that result be explained with…
A: Enzymes are biological machines that may catalyze certain processes in the cell. Enzymes catalyze…
Q: D Question 5 5. Comparisons of homologous proteins can provide information about how important an…
A: Homologous protein are the protein that is the proponent of the ancestors of both human and dog.…
Q: Which of the following molecules is NOT an amphipathic? phosphatidyl choline cholesterol…
A: Amphiphatic are the molecules which contain both hydrophobic as well as hydrophilic in their…
Q: I'm working on the thermodynamics of protein binding but I want to understand more on the enthalpic…
A: Introduction Protein folding and protein-ligand interaction are basically thermodynamically driven…
Q: Determine the number of carbon atoms present and the the total number of phosphate groups present in…
A: Glycolysis is a major metabolic pathway in the breakdown of carbohydrates such as glucose.…
Q: 2. You want to clone a specific PCR amplicon. You have determined that the amplicon you want to…
A: pUC57 is a commonly used plasmid cloning vector in E. coli.
Q: The concentration of your RNA solution is 1500 ng/μl. How much RNA solution do you need to use when…
A: The concentration of RNA in solution can be determined by measuring absorbance at 360 nm.
Q: Is it somehow possible to generate carbohydrate from non-carbohydrate? If yes, how and if no, why?
A: Carbohydrates are organic molecules, which act as the primary source of energy. Glucose is the…
Q: find stereochemistry, (from the structure) , rotational bond and geometrical / optical isomer for…
A: Phenyotin is a synthetic compound and also called Diphenylhydantoin and it is a potent…
Q: H3N- CH-C CH-CH3 CH2 CH3
A: The net charge on an amino acid is determined by the pKa of the ionizable groups on the amino acid.
Q: Which of the following statements are correct? Explain your answers.A. An atomic nucleus contains…
A: Since the atom and nucleus were discovered. The bonding of a proton and a neutron, a proton and a…
Q: Glucose is phosphorylated to glucose-6-phosphate by hexokinase in the first step of the glycolytic…
A: Reactions that have a positive value of Delta G are not favorable in nature. Hence in order to…
Q: Using a 1 cm cuvette, the absorbance at 260nm of your double-stranded DNA sample is 0.15. What is…
A: Lambert-Beer's law can be used to calculate the concentration of DNA in a sample. It states that the…
Q: During asexual reproduction, the zygote develops into an embryo which turns to be an identical copy…
A: Asexual reproduction is the process of producing identical daughter cells without fusion of gamets.…
Q: Why is DNA not RNA the storage for genetic information?
A: DNA : Deoxyribonucleic acid is a genetic material for the storage of information. The structure of…
Q: What are the structural features of 7N7H (Viperin-like enzyme)? a) Must show 3D structure
A: An enzyme is a biological catalyst. it is usually protein. 'Viperin' is a member of the radical…
Q: What is cryopreservation? How do we reduce the risk of cell damage from osmolytic pressure in…
A: Cryopreservation is primarily used to preserve biological specimens. Individual cells and biological…
Q: Calculate the overall ΔG° (report up to two decimal places) for the net reaction (see attached…
A: Standard free energy change is the Gibb's free energy at 273k temperature and 1 atm pressure ∆G°=…
Q: the enzyme and substrate should be mixed only when the reaction is initiated (when the absorbance…
A: Kinetics is the branch which deals with rate of changes. From above all given situations in the…
Q: Identify the polar and nonpolar parts of the assembly. Identify the pocket and the two bilayers that…
A: Polar components are those that are hydrophilic and soluble in water. Non polar components are those…
Q: Phospholipids have ____ fatty acids while triglycerides _____ fatty acids.
A: Lipids are defined as organic substances insoluble in water but soluble in organic solvents like…
Q: Match the following descriptions with the correct lipid-based compounds:…
A: Introduction: Lipids are a heterogeneous group of biomolecules that include fats, oils, waxes, and…
Q: Match the following methods of Analysis
A: The Biochemical analysis techniques are a set of methods, procedures and assays. This enables…
Q: Lec 3 Protein Digestion Make a flow chart of: (Indicate the organs, enzymes and how it functions)…
A: In process of digestion, complex molecules are converted to simple molecules with the…
Q: How are the organelles in a cell similar to the organs in a human body? Explain in 3-5 sentences.
A: Organelles are cellular components, which perform specific functions inside the cell. Different…
Step by step
Solved in 2 steps with 1 images
- 1.Ala-Phe-Lys-Val-Val-Glu From the above polypeptide, what amino acid/s go/goes inside the cell after the following treatment: Chemotrypsin, thermolysin, then finally pepsin. What protein is left undigested? Write the primary structure of the undigested protein? 2.K-V-F-W-P-L-A-Y a.Chemotrypsin treatment b.Trypsin treatment c.Pepsin treatment d.Thermolysin treatment 3.Total acid hydrolysis of a pentapeptide complemented by total alkalinehydrolysis yields an equimolar mixture of 5 amino acids listed alphabetically, ala-cys,lys,phe,ser. N-terminal analysis with phenylisothiocyanate (PITC) generate PTH-ser. Trypsin digestion produces a tripeptide where N-terminal residue is cys and a dipeptide with ser as its N- terminal.Chemotrypsin digestion of the above tripeptide yields ala plus another dipeptide. A.What is the amino acid sequence of the tripeptide B.What is the amino acid sequence of the dipeptide derived from trypsin digestion? C.What is the primary structure of the original…pls answer the following question. -Which amino acids contain the following?a.sulfur/sulfhydryl group b. aromatic group c. imidazole ring d. guanidine group e. indole group -Classify the following proteins according to their biological function: casein, albumin gluten, and myoglobin. -Which levels of protein structure organization are lost during hydrolysis and denaturation? -What is the Beer-Lambert's Law? Why is it relevant to the quantitative analysis of proteins? -What are the other protein components of milk aside from casein?5. protein = D-Glucosaminate-6-phosphate Ammonia-lyase Based on the structural properties of your protein above , how resistant (or sensitive) would your protein be to heat denaturation and why?
- what are the molecular way to diagnose Lactose intolerance disorder ? describe in detail about diagnostic phenomena of lactose intolerance and possible treatment.Illustrate and explain the difference between α-D-glucopyranose and β-D-glucopyranose structures.4 A I. Refer to the figure below and answer the following questions: 45 5 55 6 65 PH 7 75 8.5 0 10 B 15 20 25 30 35 Temperature (°C) 40 45 Legend: Blue - wild-type ß-galactosidase; Red - mutant ß-galactosidase a. What is the optimum pH of wild type ß-galactosidase? b. What is the optimum temperature of mutant ß-galactosidase? c. Which enzyme has the greater activity at pH 7.2? d. Which enzyme has the greater activity at a temperature of 42.5°C? e. Which enzyme has greater activity if pH decreases from 7.5 to 6.4? f. Which enzyme has greater activity if temperature increases from 40°C to 41 °C? 50 55
- describe a detailed experimental procedure for the chemical synthesis of proteins with the α-ketoacid hydroxylamine (KAHA ligation), using (S)-5-oxaproline (Opr) as a key building block.Determine the sequence of the following 10-mer of ferritin using the data below. i. Complete acid hydrolysis of the 10-mer results in identification of 1A, 1D, 1E, 1F, 1H, 1K, 2L, 1T, 1Y ii. Digestion of the 10-mer with chymotrypsin results in 3 fragments containing (D, H, K, T), (F, L) and (A, E, L, Y) iii. Digestion of the 10-mer with V8 protease, which cuts on the C-side of acidic amino acids, results in three fragments containing (D, F, L, Y), (A, E, L) and (H, K, T) iv. Digestion with trypsin results in a fragment H-T and a fragment with all the other amino acids v. Treatment of the 10-mer with FDNB followed by complete acid hydrolysis results in the following two derivatives, DNP-1 and DNP-2 vi. Treatment of the 10-mer with a carboxypeptidase results in a free threonineTreatment of a polypeptide by 2-mercaptoethanol yields two polypeptides that have the following amino acid sequences: Ala-Phe-Cys-Met-Tyr-Cys-Leu-Trp-Cys-Asn Val-Cys-Trp-Val-Ile-Phe-Gly-Cys-Lys Chymotrypsin-catalyzed hydrolysis of the intact polypeptide yields polypeptide fragments with the following amino acid compositions: (Ala, Phe) (Asn, Cys2, Met,Tyr) (Cys, Gly, Lys) (Cys2, Leu,Trp2,Val) (Ile, Phe,Val) Indicate the positions of the disulfide bonds in the original polypeptide. I don't understand how the order of amino acids in Step 2 was arranged. Please explain. Thank you.
- Treatment of a polypeptide by 2-mercaptoethanol yields two polypeptides that have the following amino acid sequences: Ala-Phe-Cys-Met-Tyr-Cys-Leu-Trp-Cys-Asn Val-Cys-Trp-Val-Ile-Phe-Gly-Cys-Lys Chymotrypsin-catalyzed hydrolysis of the intact polypeptide yields polypeptide fragments with the following amino acid compositions: (Ala, Phe) (Asn, Cys2, Met,Tyr) (Cys, Gly, Lys) (Cys2, Leu,Trp2,Val) (Ile, Phe,Val) Indicate the positions of the disulfide bonds in the original polypeptide.27. Draw palmitic acid, identify a, B and w carbons, write out its short hand notation 28. Examine the peptide sequence below and then answer the questions: H2N-Gly- Leu- Ala-Asp-Cys-Asn-Trp-lle-Ser-Phe-Lys-Cys-Arg-Pro-coOH a. Circle the asparagine residue b. A possible intramolecular disulfide bond can be formed within this peptide between which two residues? c. Circle one residue which has a positively charged side chain under pH 7.4 d. Circle one residue which has a negatively charged side chain under pH 7.4 e. Circle the residue which can be phosphorylated1. What are the effects of a) amino acid composition and sequence and b) intramolecular and intermolecular forces of attraction to protein folding? 2. What molecular property of amino acids can be used to justity the concept that the "molecular part of the protein can exhibit the same property as the molecular 'whole' (protein molecule?). Provide a comprehensive discussion using one molecular property. 3. Discuss two metabolic disorders which are caused by protein misfolding. Explain the metabolic consequence of the disorder. 4. If a non-science person asks you what protein folding is and how the concept is related to metabolic disorders, how are you going to explain the concept? (please summarize the concepts used, thank you!)