Choose among proteins A, B, C, and D. 1. Protein that will most strongly bind to an anion exchange colum. 2. Protein that will elute last in gel filtration chromatography. 3. Protein that will elute first in hydrophobic interaction chromatography. 4. Protein that will elute last in a carbohydrate containing colum.
Choose among proteins A, B, C, and D. 1. Protein that will most strongly bind to an anion exchange colum. 2. Protein that will elute last in gel filtration chromatography. 3. Protein that will elute first in hydrophobic interaction chromatography. 4. Protein that will elute last in a carbohydrate containing colum.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Choose among proteins A, B, C, and D.
1. Protein that will most strongly bind to an anion exchange colum.
2. Protein that will elute last in gel filtration chromatography.
3. Protein that will elute first in hydrophobic interaction chromatography.
4. Protein that will elute last in a carbohydrate containing colum.
Transcribed Image Text:Consider a mixture comprised of the following proteins below:
MW Charge
Affinity to a
Protein
%Polarity
(kDa) (pH 7.4) specific ligand
A
760
-3
None
45
B
300
+1
Carbohydrate
70
C
80
-1
None
60
55
+2
None
25
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